Proteomic Studies on the Effects of Lipo-Chitooligosaccharide and Thuricin 17 under Unstressed and Salt Stressed Conditions in Arabidopsis thaliana.

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Compatible plant-microbe interactions favor plant growth and development, and help plants deal with these environmental challenges. Microorganisms produce a diverse range of elicitor molecules to establish symbiotic relationships with the plants they associate with, in a given ecological niche. Lipo-chitooligosaccharide (LCO) and Thuricin 17 (Th17) are two such compounds shown to positively influence plant growth of both legumes and non-legumes. Arabidopsis thaliana responded positively to treatment with the bacterial signal compounds LCO and Th17 in the presence of salt stress (up to 250 mM NaCl). Shotgun proteomics of unstressed and 250 mM NaCl stressed A. thaliana rosettes (7 days post stress) in combination with the LCO and Th17 revealed many known, putative, hypothetical, and unknown proteins. Overall, carbon and energy metabolic pathways were affected under both unstressed and salt stressed conditions when treated with these signals. PEP carboxylase, Rubisco-oxygenase large subunit, pyruvate kinase, and proteins of photosystems I and II were some of the noteworthy proteins enhanced by the signals, along with other stress related proteins. These findings suggest that the proteome of A. thaliana rosettes is altered by the bacterial signals tested, and more so under salt stress, thereby imparting a positive effect on plant growth under high salt stress. The roles of the identified proteins are discussed here in relation to salt stress adaptation, which, when translated to field grown crops can be a crucial component and of significant importance in agriculture and global food production. The mass spectrometry proteomics data have been deposited to the ProteomeXchange with identifier PXD004742.", "claims": [{"id": "claim-method", "statement": "The treatment contrasts were then analyzed for fold-change after normalization, and Fisher’s Exact test was used to narrow down the up- and down-regulated proteins, to predict their probable functions at 24 h after signal compound treatment and 7 days after NaCl stress imposition.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27625672", "locator": "sections[14] \"Protein Profiling\" paragraph 2", "quote": "The treatment contrasts were then analyzed for fold-change after normalization, and Fisher’s Exact test was used to narrow down the up- and down-regulated proteins, to predict their probable functions at 24 h after signal compound treatment and 7 days after NaCl stress imposition."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium1 via the PRIDE partner repository (Vizcaino et al., 2013) with the dataset identifier PXD004742.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27625672", "locator": "sections[11] \"Data Analysis\" paragraph 3", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium1 via the PRIDE partner repository (Vizcaino et al., 2013) with the dataset identifier PXD004742."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper reports that LCO and Th17 affected carbon and energy metabolic pathways under unstressed and salt-stressed conditions. 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