Global proteomics dataset of miR-126 overexpression in acute myeloid leukemia.

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Leukemia cells were transduced either with an empty control lentivirus or one containing the sequence for miR-126, and resulting cells were analyzed using ultra-high performance liquid chromatography (UHPLC) coupled with high resolution mass spectrometry. The mass spectrometry data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD001994. The proteomics data and statistical analysis described in this article is associated with a research article, \"miR-126 regulates distinct self-renewal outcomes in normal and malignant hematopoietic stem cells\" (Lechman et al., 2016) [1], and serves as a resource for researchers working in the field of microRNAs and their regulation of protein levels.", "claims": [{"id": "claim-method", "statement": "Protein ratios for each biological replicate were calculated, and this final table was processed in Limma (R Statistical Framework [6]) to determine those proteins that are significantly regulated according to the moderated t-test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27656662", "locator": "sections[6] \"Label-free quantitative proteomics analysis\" paragraph 3", "quote": "Protein ratios for each biological replicate were calculated, and this final table was processed in Limma (R Statistical Framework [6]) to determine those proteins that are significantly regulated according to the moderated t-test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data is within this article and the mass spectrometry data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE:PXD001994 (http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD001994)", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27656662", "locator": "sections[1] \"p\" paragraph 1", "quote": "Data is within this article and the mass spectrometry data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE:PXD001994 (http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD001994)"}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper used Limma to determine significantly regulated proteins, and the mass spectrometry source data were deposited in the ProteomeXchange Consortium via the PRIDE partner repository under PRIDE: PXD001994.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:27656662", "locator": "sections[3] \"Data\" paragraph 2", "quote": "The data files shared here provide the computational workflow that was applied to filter the data in Perseus [2], and to determine significantly regulated proteins using Limma [6]."}, {"asset": "source:pmid:27656662", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The mass spectrometry data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD001994."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The paper used Limma to determine significantly regulated proteins, and the mass spectrometry source data were deposited in the ProteomeXchange Consortium via the PRIDE partner repository under PRIDE: PXD001994.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:27656662"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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