Behavioral and omics analyses study on potential involvement of dipeptide balenine through supplementation in diet of senescence-accelerated mouse prone 8.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:27672559", "kind": "science-record", "revision": 8, "content_hash": "sha256:14dd2686f8bd6acb6cf3f44885ab1890931d22735a423b0def01042367fddf20", "created_at": "2026-11-01T15:40:20Z", "updated_at": "2026-11-01T22:42:47Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Behavioral and omics analyses study on potential involvement of dipeptide balenine through supplementation in diet of senescence-accelerated mouse prone 8.", "doi": "10.1016/j.gdata.2016.09.004", "pmid": "27672559", "authors": ["Nobuhiro Wada", "Satoru Yamanaka", "Junko Shibato", "Randeep Rakwal", "Satoshi Hirako", "Yuzuru Iizuka", "Hyounju Kim", "Akiyo Matsumoto", "Ai Kimura", "Fumiko Takenoya", "Genta Yasunaga", "Seiji Shioda"], "journal": "Genomics data", "year": 2016, "abstract": "This study investigates effects of dipeptide balenine, as a major component of whale meat extract (hereafter, WME), supplementation on senescence-accelerated mouse prone 8 (SAMP8), an Alzheimer's disease (AD) model at level of learning and memory formation and brain expression profiles genome-wide in brain. Mice fed experimental balenine (+ WME) supplemented diet for 26 weeks were subjected to four behavioral tests - open field, Y-maze, new object recognition, and water-filled multiple T-maze - to examine effects on learning and memory. Brain transcriptome of SAMP8 mice-fed the WME diet over control low-safflower oil (LSO) diet-fed mice was delineated on a 4 × 44 K mouse whole genome DNA microarray chip. Results revealed the WME diet not only induced improvements in the learning and memory formation but also positively modulated changes in the brain of the SAMP8 mouse; the gene inventories are publically available for analysis by the scientific community. Interestingly, the SAMP8 mouse model presented many genetic characteristics of AD, and numerous novel molecules (Slc2a5, Treh, Fbp1, Aldob, Ppp1r1a, DNase1, Agxt2l1, Cyp2e1, Acsm1, Acsm2, and Pah) were revealed over the SAMR1 (senescence-accelerated mouse resistant 1) mouse, to be oppositely regulated/recovered under the balenine (+ WME) supplemented diet regime by DNA microarray and bioinformatics analyses. Our present study demonstrates an experimental strategy to understand the effects of dipeptide balenine, prominetly contained in meat diet, on SAMP8, providing new insight into whole brain transcriptome changes genome-wide. The gene expression data has been deposited into the Gene Expression Omnibus (GEO): GSE76459. The data will be a valuable resource in examining the effects of natural products, and which could also serve as a human model for further functional analysis and investigation.", "claims": [{"id": "claim-method", "statement": "Differences between three groups were assessed using the one-way analysis of variance (ANOVA) followed by Bonferroni's post-hoc test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27672559", "locator": "sections[16] \"Statistical analysis\" paragraph 2", "quote": "Differences between three groups were assessed using the one-way analysis of variance (ANOVA) followed by Bonferroni's post-hoc test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The outputs of microarray analysis used in this study are available under the series number GSE 76459 (whole genome - http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE76459; at the NCBI Gene Expression Omnibus (GEO) public functional genomics data repository (http://www.ncbi.nlm.nih.gov/geo/info/linking.html).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27672559", "locator": "sections[21] \"Access to gene array data\" paragraph 2", "quote": "The outputs of microarray analysis used in this study are available under the series number GSE 76459 (whole genome - http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE76459; at the NCBI Gene Expression Omnibus (GEO) public functional genomics data repository (http://www.ncbi.nlm.nih.gov/geo/info/linking.html)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper used one-way ANOVA followed by Bonferroni’s post-hoc test, with statistical significance defined as p < 0.05; its source gene-expression data are available through GEO under accession GSE76459. 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