Immunoseroproteomic Profiling in African American Men with Prostate Cancer: Evidence for an Autoantibody Response to Glycolysis and Plasminogen-Associated Proteins.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:27742740", "kind": "science-record", "revision": 3, "content_hash": "sha256:ddaf1089a8195d21a620de9f5e3a9387db1a7466262907ac028660e3d946f475", "created_at": "2026-11-01T15:40:20Z", "updated_at": "2026-11-01T21:20:41Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Immunoseroproteomic Profiling in African American Men with Prostate Cancer: Evidence for an Autoantibody Response to Glycolysis and Plasminogen-Associated Proteins.", "doi": "10.1074/mcp.M116.060244", "pmid": "27742740", "authors": ["Tino W Sanchez", "Guangyu Zhang", "Jitian Li", "Liping Dai", "Saied Mirshahidi", "Nathan R Wall", "Clayton Yates", "Colwick Wilson", "Susanne Montgomery", "Jian-Ying Zhang", "Carlos A Casiano"], "journal": "Molecular & cellular proteomics : MCP", "year": 2016, "abstract": "African American (AA) men suffer from a disproportionately high incidence and mortality of prostate cancer (PCa) compared with other racial/ethnic groups. Despite these disparities, African American men are underrepresented in clinical trials and in studies on PCa biology and biomarker discovery. We used immunoseroproteomics to profile antitumor autoantibody responses in AA and European American (EA) men with PCa, and explored differences in these responses. This minimally invasive approach detects autoantibodies to tumor-associated antigens that could serve as clinical biomarkers and immunotherapeutic agents. Sera from AA and EA men with PCa were probed by immunoblotting against PC3 cell proteins, with AA sera showing stronger immunoreactivity. Mass spectrometry analysis of immunoreactive protein spots revealed that several AA sera contained autoantibodies to a number of proteins associated with both the glycolysis and plasminogen pathways, particularly to alpha-enolase (ENO1). The proteomic data is deposited in ProteomeXchange with identifier PXD003968. Analysis of sera from 340 racially diverse men by enzyme-linked immunosorbent assays (ELISA) showed higher frequency of anti-ENO1 autoantibodies in PCa sera compared with control sera. We observed differences between AA-PCa and EA-PCa patients in their immunoreactivity against ENO1. Although EA-PCa sera reacted with higher frequency against purified ENO1 in ELISA and recognized by immunoblotting the endogenous cellular ENO1 across a panel of prostate cell lines, AA-PCa sera reacted weakly against this protein by ELISA but recognized it by immunoblotting preferentially in metastatic cell lines. These race-related differences in immunoreactivity to ENO1 could not be accounted by differential autoantibody recognition of phosphoepitopes within this antigen. Proteomic analysis revealed differences in the posttranslational modification profiles of ENO1 variants differentially recognized by AA-PCa and EA-PCa sera. These intriguing results suggest the possibility of race-related differences in the antitumor autoantibody response in PCa, and have implications for defining novel biological determinants of PCa health disparities.", "claims": [{"id": "claim-target-result", "statement": "The paper’s headline result was a higher frequency of anti-ENO1 autoantibodies in prostate cancer sera than in control sera. The abstract does not state a statistical method or statistical test; the source data were sera from 340 racially diverse men analyzed by ELISA, and the proteomic data were deposited in ProteomeXchange under PXD003968.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:27742740", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Analysis of sera from 340 racially diverse men by enzyme-linked immunosorbent assays (ELISA) showed higher frequency of anti-ENO1 autoantibodies in PCa sera compared with control sera."}, {"asset": "source:pmid:27742740", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The proteomic data is deposited in ProteomeXchange with identifier PXD003968."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The paper’s headline result was a higher frequency of anti-ENO1 autoantibodies in prostate cancer sera than in control sera. The abstract does not state a statistical method or statistical test; the source data were sera from 340 racially diverse men analyzed by ELISA, and the proteomic data were deposited in ProteomeXchange under PXD003968.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:27742740"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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