Proteome data from a host-pathogen interaction study with Staphylococcus aureus and human lung epithelial cells.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:27761493", "kind": "science-record", "revision": 5, "content_hash": "sha256:5ee7366cb0889a07d266977716f4b9d21924397f16dabd502b2a2e1b430c3831", "created_at": "2026-11-01T15:40:21Z", "updated_at": "2026-11-01T18:48:24Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Proteome data from a host-pathogen interaction study with Staphylococcus aureus and human lung epithelial cells.", "doi": "10.1016/j.dib.2016.03.027", "pmid": "27761493", "authors": ["Kristin Surmann", "Marjolaine Simon", "Petra Hildebrandt", "Henrike Pförtner", "Stephan Michalik", "Vishnu M Dhople", "Barbara M Bröker", "Frank Schmidt", "Uwe Völker"], "journal": "Data in brief", "year": 2016, "abstract": "To simultaneously obtain proteome data of host and pathogen from an internalization experiment, human alveolar epithelial A549 cells were infected with Staphylococcus aureus HG001 which carried a plasmid (pMV158GFP) encoding a continuously expressed green fluorescent protein (GFP). Samples were taken hourly between 1.5 h and 6.5 h post infection. By fluorescence activated cell sorting GFP-expressing bacteria could be enriched from host cell debris, but also infected host cells could be separated from those which did not carry bacteria after contact (exposed). Additionally, proteome data of A549 cells which were not exposed to S. aureus but underwent the same sample processing steps are provided as a control. Time-resolved changes in bacterial protein abundance were quantified in a label-free approach. Proteome adaptations of host cells were monitored by comparative analysis to a stable isotope labeled cell culture (SILAC) standard. Proteins were extracted from the cells, digested proteolytically, measured by nanoLC-MS/MS, and subsequently identified by database search and then quantified. The data presented here are related to a previously published research article describing the interplay of S. aureus HG001 and human epithelial cells (Surmann et al., 2015 [1]). They have been deposited to the ProteomeXchange platform with the identifiers PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD002384 for the S. aureus HG001 proteome dataset and PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD002388 for the A549 proteome dataset.", "claims": [{"id": "claim-data-access", "statement": "The data presented here are related to a previously published research article describing the interplay of S. aureus HG001 and human epithelial cells (Surmann et al., 2015 [1]). 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They have been deposited to the ProteomeXchange platform with the identifiers PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD002384 for the S. aureus HG001 proteome dataset and PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD002388 for the A549 proteome dataset."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Time-resolved changes in bacterial protein abundance were quantified in a label-free approach, while host-cell proteome adaptations were assessed by comparative analysis with a SILAC standard.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:27761493", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Time-resolved changes in bacterial protein abundance were quantified in a label-free approach."}, {"asset": "source:pmid:27761493", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Proteome adaptations of host cells were monitored by comparative analysis to a stable isotope labeled cell culture (SILAC) standard."}, {"asset": "source:pmid:27761493", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "They have been deposited to the ProteomeXchange platform with the identifiers PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD002384 for the S. aureus HG001 proteome dataset and PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD002388 for the A549 proteome dataset."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Time-resolved changes in bacterial protein abundance were quantified in a label-free approach, while host-cell proteome adaptations were assessed by comparative analysis with a SILAC standard.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:27761493"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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