Dataset of the Botrytis cinerea phosphoproteome induced by different plant-based elicitors.

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The data presented in this article provided a differential mass spectrometry-based analysis of the phosphoproteome of B. cinerea under two different phenotypical conditions induced by the use of two different elicitors: glucose and deproteinized Tomate Cell Walls (TCW). A total 1138 and 733 phosphoproteins were identified for glucose and TCW culture conditions respectively. Raw data are deposited at the ProteomeXchange Consortium via the PRIDE partner repository with the data set identifier (PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD003099). Further interpretation and discussion of these data are provided in our research article entitled \"Phosphoproteome analysis of B.cinerea in response to different plant-based elicitors\" (Liñeiro et al., 2016) [1].", "claims": [{"id": "claim-method", "statement": "The maximum number of missed cleavages was set at 3, and peptides were filtered based on an false discovery rate (FDR) lower than 1%.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27761508", "locator": "sections[10] \"LC-MS/MS and data analysis\" paragraph 2", "quote": "The maximum number of missed cleavages was set at 3, and peptides were filtered based on an false discovery rate (FDR) lower than 1%."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Raw LC-MS/MS files are also available via ProteomeXchange Consortium31 via the PRIDE accession number (PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD003099).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27761508", "locator": "sections[5] \"Data\" paragraph 2", "quote": "Raw LC-MS/MS files are also available via ProteomeXchange Consortium31 via the PRIDE accession number (PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD003099)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper identified 1,138 and 733 phosphoproteins under glucose and TCW conditions, respectively, filtered protein identifications using a false discovery rate below 1% and requiring identification in at least 3 of 4 biological replicates per condition, and deposited the raw LC-MS/MS data through ProteomeXchange via PRIDE.", "kind": "quantitative", "target": {"value": 1.0, "unit": "per cent", "tolerance": null, "source": "Peptide tolerance was set at 7 ppm in MS and at 0.5 Da in MS/MS mode. The maximum number of missed cleavages was set at 3, and peptides were filtered based on an false discovery rate (FDR) lower than 1%. For comparative analysis only those proteins identified in at least 3 of the 4 biological samples run per assayed condition were consider."}, "evidence": [{"asset": "source:pmid:27761508", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "A total 1138 and 733 phosphoproteins were identified for glucose and TCW culture conditions respectively."}, {"asset": "source:pmid:27761508", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Raw data are deposited at the ProteomeXchange Consortium via the PRIDE partner repository with the data set identifier (PRIDE: http://www.ebi.ac.uk/pride/archive/projects/PXD003099)."}, {"asset": "source:pmid:27761508", "locator": "sections[10] \"LC-MS/MS and data analysis\" paragraph 2", "quote": "Peptide tolerance was set at 7 ppm in MS and at 0.5 Da in MS/MS mode. 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