KCNJ3 is a new independent prognostic marker for estrogen receptor positive breast cancer patients.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:27835900", "kind": "science-record", "revision": 8, "content_hash": "sha256:aeffdc256d077385940a76bad824ed3d0872749afa0cb7a70c63766f03c0b4ca", "created_at": "2026-11-01T15:40:21Z", "updated_at": "2026-11-01T21:59:12Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "KCNJ3 is a new independent prognostic marker for estrogen receptor positive breast cancer patients.", "doi": "10.18632/oncotarget.13224", "pmid": "27835900", "authors": ["Sarah Kammerer", "Armin Sokolowski", "Hubert Hackl", "Dieter Platzer", "Stephan Wenzel Jahn", "Amin El-Heliebi", "Daniela Schwarzenbacher", "Verena Stiegelbauer", "Martin Pichler", "Simin Rezania", "Heidelinde Fiegl", "Florentia Peintinger", "Peter Regitnig", "Gerald Hoefler", "Wolfgang Schreibmayer", "Thomas Bauernhofer"], "journal": "Oncotarget", "year": 2016, "abstract": "Numerous studies showed abnormal expression of ion channels in different cancer types. Amongst these, the potassium channel gene KCNJ3 (encoding for GIRK1 proteins) has been reported to be upregulated in tumors of patients with breast cancer and to correlate with positive lymph node status. We aimed to study KCNJ3 levels in different breast cancer subtypes using gene expression data from the TCGA, to validate our findings using RNA in situ hybridization in a validation cohort (GEO ID GSE17705), and to study the prognostic value of KCNJ3using survival analysis. In a total of > 1000 breast cancer patients of two independent data sets we showed a) that KCNJ3 expression is upregulated in tumor tissue compared to corresponding normal tissue (p < 0.001), b) that KCNJ3 expression is associated with estrogen receptor (ER) positive tumors (p < 0.001), but that KCNJ3 expression is variable within this group, and c) that ER positive patients with high KCNJ3 levels have worse overall (p < 0.05) and disease free survival probabilities (p < 0.01), whereby KCNJ3 is an independent prognostic factor (p <0.05). In conclusion, our data suggest that patients with ER positive breast cancer might be stratified into high risk and low risk groups based on the KCNJ3 levels in the tumor.", "claims": [{"id": "claim-method", "statement": "A time-independent Cox-proportional hazard approach was applied for univariate and multivariate survival analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27835900", "locator": "sections[16] \"Statistical analysis\" paragraph 2", "quote": "A time-independent Cox-proportional hazard approach was applied for univariate and multivariate survival analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The gene expression levels (RNAseq V2 level 3 data) of 950 invasive breast carcinoma samples and of 105 samples from corresponding healthy tissues were downloaded from the The Cancer Genome Atlas (TCGA) data portal (https://tcga-data.nci.nih.gov) and the upper quartile normalized counts from the RSEM pipeline were used.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27835900", "locator": "sections[9] \"Gene expression data from the cancer genome atlas\" paragraph 2", "quote": "The gene expression levels (RNAseq V2 level 3 data) of 950 invasive breast carcinoma samples and of 105 samples from corresponding healthy tissues were downloaded from the The Cancer Genome Atlas (TCGA) data portal (https://tcga-data.nci.nih.gov) and the upper quartile normalized counts from the RSEM pipeline were used."}], "state": "complete"}, {"id": "claim-target-result", "statement": "In ER-positive breast cancer patients, high KCNJ3 expression is associated with worse overall and disease-free survival, with KCNJ3 serving as an independent prognostic factor.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:27835900", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We aimed to study KCNJ3 levels in different breast cancer subtypes using gene expression data from the TCGA, to validate our findings using RNA in situ hybridization in a validation cohort (GEO ID GSE17705), and to study the prognostic value of KCNJ3 using survival analysis."}, {"asset": "source:pmid:27835900", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "In a total of > 1000 breast cancer patients of two independent data sets we showed a) that KCNJ3 expression is upregulated in tumor tissue compared to corresponding normal tissue (p < 0.001), b) that KCNJ3 expression is associated with estrogen receptor (ER) positive tumors (p < 0.001), but that KCNJ3 expression is variable within this group, and c) that ER positive patients with high KCNJ3 levels have worse overall (p < 0.05) and disease free survival probabilities (p < 0.01), whereby KCNJ3 is an independent prognostic factor (p <0.05)."}, {"asset": "source:pmid:27835900", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "In conclusion, our data suggest that patients with ER positive breast cancer might be stratified into high risk and low risk groups based on the KCNJ3 levels in the tumor."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "In ER-positive breast cancer patients, high KCNJ3 expression is associated with worse overall and disease-free survival, with KCNJ3 serving as an independent prognostic factor.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:27835900"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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