Curcumin increases exosomal TCF21 thus suppressing exosome-induced lung cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:27894084", "kind": "science-record", "revision": 8, "content_hash": "sha256:a07425ff8f053add4697272e77d467d64a7ac3ad3b2c1ebca07e5344d21f5ed6", "created_at": "2026-11-01T15:40:21Z", "updated_at": "2026-11-01T21:59:12Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Curcumin increases exosomal TCF21 thus suppressing exosome-induced lung cancer.", "doi": "10.18632/oncotarget.13499", "pmid": "27894084", "authors": ["Hao Wu", "Jingcheng Zhou", "Chao Zeng", "Da Wu", "Zhimin Mu", "Baokun Chen", "Yuancai Xie", "Yiwang Ye", "Jixian Liu"], "journal": "Oncotarget", "year": 2016, "abstract": "Curcumin is a novel drug for lung cancer treatment. However, the mechanism underlying the anti-tumor effect of curcumin remains elusive. Previous evidences indicated that, the methylating transferase DNMT1 is downregulated by curcumin, and the transcription factor 21 (TCF21) is suppressed by DNMT1. We hereby attempt to elucidate the correlation between curcumin treatment and TCF21 expression. Exosomes derived from curcumin-pretreated H1299 cells were used to treat BEAS-2B cells, which induced proliferation, colony formation and migration of BEAS-2B cells. An increase in TCF21 expression in response to curcumin was also seen, as revealed by real-time PCR (RT-PCR) and western blot. Analysis using the GEO database (access #GSE21210) indicated that a positive correlation existed between TCF21 levels and lung cancer patient survival. TCF21 overexpression and knockdown was introduced to H1299 cells through lentiviral system, which led to suppression and promotion of tumor growth, respectively. We also demonstrated that DNMT1 expression was downregulated by curcumin. Therefore, curcumin exerts its anti-cancer function by downregulating DNMT1, thereby upregulating TCF21.", "claims": [{"id": "claim-method", "statement": "Data were represented as mean±SD, and analyzed using Student's t-test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27894084", "locator": "sections[19] \"Statistical analysis\" paragraph 2", "quote": "Data were represented as mean±SD, and analyzed using Student's t-test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The dataset with access number of GSE21210, which contains a larger cohort of lung cancer patients (n=102), was used for data analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27894084", "locator": "sections[15] \"GEO database analysis\" paragraph 2", "quote": "The dataset with access number of GSE21210, which contains a larger cohort of lung cancer patients (n=102), was used for data analysis."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper analyzed all experiments in triplicates unless otherwise stated, represented data as mean±SD, and used Student’s t-test, with differences considered significant at p<0.05. The source data were obtained from the NCBI GEO dataset GSE21210, containing 102 lung cancer patients.", "kind": "quantitative", "target": {"value": 0.05, "unit": "p-value", "tolerance": null, "source": "All experiments were performed in triplicates unless otherwise stated. Data were represented as mean±SD, and analyzed using Student's t-test. Differences between groups were considered statistically significant if p<0.05."}, "evidence": [{"asset": "source:pmid:27894084", "locator": "sections[19] \"Statistical analysis\" paragraph 2", "quote": "All experiments were performed in triplicates unless otherwise stated. Data were represented as mean±SD, and analyzed using Student's t-test. Differences between groups were considered statistically significant if p<0.05."}, {"asset": "source:pmid:27894084", "locator": "sections[15] \"GEO database analysis\" paragraph 2", "quote": "The Gene Expression Omnibus (GEO) database from the National Center for Biotechnology Information (NCBI) was used to confirm that TCF21 downregulation is a signature for lung cancer patients with a poor survival. The dataset with access number of GSE21210, which contains a larger cohort of lung cancer patients (n=102), was used for data analysis."}, {"asset": "source:pmid:27894084", "locator": "sections[5] \"TCF21 is a positive biomarker in lung cancer prognosis\" paragraph 2", "quote": "Clearly, low TCF21 expression is associated with poor survival of lung cancer (Figure 3A)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The paper analyzed all experiments in triplicates unless otherwise stated, represented data as mean±SD, and used Student’s t-test, with differences considered significant at p<0.05. 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