SWATH-MS dataset of heat-shock treated Drosophila melanogaster embryos.

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Here we applied a DIA approach (termed SWATH) to monitor the dynamics of the Drosophila melanogaster embryonic proteome upon heat-shock treatment. Embryos were incubated for 0.5, 1 or 3 h at 37 °C to induce heat-shock or maintained at 25 °C. The present dataset contains SWATH files acquired on a Sciex Triple-TOF 6600. A spectral library built in-house was used to analyse these data and led to the quantification of more than 2500 proteins at every timepoint. The files presented here are permanent digital maps and can be reanalysed to search for new questions. The data have been deposited with the ProteomeXchange Consortium with the dataset identifier PRIDE: PXD004753.", "claims": [{"id": "claim-data-access", "statement": "All the mass spectrometry data have been deposited with the ProteomeXchange [7] Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD004753.\nThe results from the SWATH analysis of the heat-shock time course experiment are provided in Supplementary Table.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:27900350", "locator": "sections[10] \"Data availability\" paragraphs 1-3", "quote": "All the mass spectrometry data have been deposited with the ProteomeXchange [7] Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD004753.\nThe results from the SWATH analysis of the heat-shock time course experiment are provided in Supplementary Table."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The in-house spectral-library SWATH analysis quantified more than 2500 proteins at every heat-shock time point, and the mass-spectrometry data are accessible through ProteomeXchange via PRIDE under PXD004753.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:27900350", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "A spectral library built in-house was used to analyse these data and led to the quantification of more than 2500 proteins at every timepoint."}, {"asset": "source:pmid:27900350", "locator": "sections[10] \"Data availability\" paragraph 2", "quote": "All the mass spectrometry data have been deposited with the ProteomeXchange [7] Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD004753."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The in-house spectral-library SWATH analysis quantified more than 2500 proteins at every heat-shock time point, and the mass-spectrometry data are accessible through ProteomeXchange via PRIDE under PXD004753.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:27900350"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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