Transcriptomes of bovine ovarian follicular and luteal cells.

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The normalized linear microarray data was deposited to the NCBI GEO repository (GSE83524). Subsequent ANOVA determined genes that were enriched (≥2 fold more) or decreased (≤-2 fold less) in one cell type compared to all three other cell types, and these analyzed and filtered datasets are presented as tables. Genes that were shared in enriched expression in both follicular cell types (GCs and TCs) or in both luteal cells types (LLCs and SLCs) are also reported in tables. The standard deviation of the analyzed array data in relation to the log of the expression values is shown as a figure. These data have been further analyzed and interpreted in the companion article \"Gene expression profiling of ovarian follicular and luteal cells provides insight into cellular identities and functions\" (Romereim et al., 2017) [1].", "claims": [{"id": "claim-method", "statement": "Array analysis was then performed using the National Institute of Aging tool (http://lgsun.grc.nia.nih.gov/ANOVA/) for Analysis of Variance (ANOVA).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28004024", "locator": "sections[4] \"Experimental design, materials and methods\" paragraph 4", "quote": "Array analysis was then performed using the National Institute of Aging tool (http://lgsun.grc.nia.nih.gov/ANOVA/) for Analysis of Variance (ANOVA)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data accessibilityFiltered dataset is present as tables in this article, raw data is in the public NCBI repository GEOGSE83524. http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE83524", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28004024", "locator": "sections[1] \"p\" paragraph 1", "quote": "Data accessibilityFiltered dataset is present as tables in this article, raw data is in the public NCBI repository GEOGSE83524. http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE83524"}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper used ANOVA to identify genes enriched or decreased by at least twofold among four ovarian somatic cell types, and deposited the normalized linear microarray source data in NCBI GEO under accession GSE83524.", "kind": "quantitative", "target": {"value": 2.0, "unit": "fold change", "tolerance": null, "source": "Subsequent ANOVA determined genes that were enriched (≥2 fold more) or decreased (≤−2 fold less) in one cell type compared to all three other cell types, and these analyzed and filtered datasets are presented as tables."}, "evidence": [{"asset": "source:pmid:28004024", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The normalized linear microarray data was deposited to the NCBI GEO repository (GSE83524)."}, {"asset": "source:pmid:28004024", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Subsequent ANOVA determined genes that were enriched (≥2 fold more) or decreased (≤−2 fold less) in one cell type compared to all three other cell types, and these analyzed and filtered datasets are presented as tables."}, {"asset": "source:pmid:28004024", "locator": "sections[4] \"Experimental design, materials and methods\" paragraph 4", "quote": "Array analysis was then performed using the National Institute of Aging tool (http://lgsun.grc.nia.nih.gov/ANOVA/) for Analysis of Variance (ANOVA)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The paper used ANOVA to identify genes enriched or decreased by at least twofold among four ovarian somatic cell types, and deposited the normalized linear microarray source data in NCBI GEO under accession GSE83524.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:28004024"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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