CD5 molecule-like and transthyretin as putative biomarkers of chronic myeloid leukemia - an insight from the proteomic analysis of human plasma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:28117336", "kind": "science-record", "revision": 7, "content_hash": "sha256:275356f0840a5c60efe81f3992f690d4fe270264dadf409b52fdbf4329450d8d", "created_at": "2026-11-01T15:40:22Z", "updated_at": "2026-11-01T21:59:15Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "CD5 molecule-like and transthyretin as putative biomarkers of chronic myeloid leukemia - an insight from the proteomic analysis of human plasma.", "doi": "10.1038/srep40943", "pmid": "28117336", "authors": ["Iram Fatima", "Saima Sadaf", "Syed Ghulam Musharraf", "Naghma Hashmi", "Muhammad Waheed Akhtar"], "journal": "Scientific reports", "year": 2017, "abstract": "Better and sensitive biomarkers are needed to help understand the mechanism of disease onset, progression, prognosis and monitoring of the therapeutic response. Aim of this study was to identify the candidate circulating markers of chronic-phase chronic myeloid leukemia (CP-CML) manifestations, having potential to develop into predictive- or monitoring-biomarkers. A proteomic approach, two-dimensional gel electrophoresis in conjunction with mass spectrometry (2DE-MS), was employed for this purpose. Based on the spot intensity measurements, six proteins were found to be consistently dysregulated in CP-CML subjects compared to the healthy controls [false discovery rate (FDR) threshold ≤0.05]. These were identified as α-1-antichymotrypsin, α-1-antitrypsin, CD5 molecule-like, stress-induced phosphoprotein 1, vitamin D binding protein isoform 1 and transthyretin by MS analysis [PMF score ≥79; data accessible via ProteomeXchange with identifier PXD002757]. Quantitative ELISA, used for validation of candidate proteins both in the pre-treated and nilotinib-treated CP-CML cases, demonstrate that CD5 molecule-like, transthyretin and alpha-1-antitrypsin may serve as useful predictive markers and aid in monitoring the response of TKI-based therapy (ANOVA p < 0.0001). Two of the circulating marker proteins, identified in this study, had not previously been associated with chronic- or acute-phase myeloid leukemia. Exploration of their probable association with CP-CML, in a larger study cohort, may add to our understanding of the disease mechanism besides developing clinically useful biomarkers in future.", "claims": [{"id": "claim-method", "statement": "The spot intensity differences obtained from the 2D-gel images of at least 3 different sets of independent plasma samples were analyzed by non-parametric Mann-Whitney U test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28117336", "locator": "sections[14] \"Statistical analysis and validation of biomarker candidates\" paragraph 2", "quote": "The spot intensity differences obtained from the 2D-gel images of at least 3 different sets of independent plasma samples were analyzed by non-parametric Mann-Whitney U test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The proteomics data was deposited to the ProteomeXchange Consortium8 via PRIDE partner repository (http://www.ebi.ac.uk/pride/) with the dataset identifier PXD002757.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28117336", "locator": "sections[5] \"2D proteome profiling and mass spectrometric analysis\" paragraph 2", "quote": "The proteomics data was deposited to the ProteomeXchange Consortium8 via PRIDE partner repository (http://www.ebi.ac.uk/pride/) with the dataset identifier PXD002757."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study used two-dimensional gel electrophoresis with mass spectrometry (2DE-MS), identified differentially expressed proteins using a false discovery rate threshold ≤0.05 and peptide mass fingerprinting score ≥79, and made the proteomics data accessible through ProteomeXchange under identifier PXD002757.", "kind": "quantitative", "target": {"value": 0.0001, "unit": "p-value", "tolerance": null, "source": "Quantitative ELISA, used for validation of candidate proteins both in the pre-treated and nilotinib-treated CP-CML cases, demonstrate that CD5 molecule-like, transthyretin and alpha-1-antitrypsin may serve as useful predictive markers and aid in monitoring the response of TKI-based therapy (ANOVA p < 0.0001)."}, "evidence": [{"asset": "source:pmid:28117336", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "A proteomic approach, two-dimensional gel electrophoresis in conjunction with mass spectrometry (2DE-MS), was employed for this purpose."}, {"asset": "source:pmid:28117336", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Based on the spot intensity measurements, six proteins were found to be consistently dysregulated in CP-CML subjects compared to the healthy controls [false discovery rate (FDR) threshold ≤0.05]."}, {"asset": "source:pmid:28117336", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "These were identified as α-1-antichymotrypsin, α-1-antitrypsin, CD5 molecule-like, stress-induced phosphoprotein 1, vitamin D binding protein isoform 1 and transthyretin by MS analysis [PMF score ≥79; data accessible via ProteomeXchange with identifier PXD002757]."}, {"asset": "source:pmid:28117336", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Quantitative ELISA, used for validation of candidate proteins both in the pre-treated and nilotinib-treated CP-CML cases, demonstrate that CD5 molecule-like, transthyretin and alpha-1-antitrypsin may serve as useful predictive markers and aid in monitoring the response of TKI-based therapy (ANOVA p < 0.0001)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The study used two-dimensional gel electrophoresis with mass spectrometry (2DE-MS), identified differentially expressed proteins using a false discovery rate threshold ≤0.05 and peptide mass fingerprinting score ≥79, and made the proteomics data accessible through ProteomeXchange under identifier PXD002757.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:28117336"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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