Proteomic Analysis of Post-synaptic Density Fractions from Shank3 Mutant Mice Reveals Brain Region Specific Changes Relevant to Autism Spectrum Disorder.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:28261056", "kind": "science-record", "revision": 6, "content_hash": "sha256:79bdc92b0fdd6afa1d0413a6809eddbc92d6976fab24d6c4ab6326333e6548af", "created_at": "2026-11-01T15:40:24Z", "updated_at": "2026-11-01T21:20:51Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Proteomic Analysis of Post-synaptic Density Fractions from Shank3 Mutant Mice Reveals Brain Region Specific Changes Relevant to Autism Spectrum Disorder.", "doi": "10.3389/fnmol.2017.00026", "pmid": "28261056", "authors": ["Dominik Reim", "Ute Distler", "Sonja Halbedl", "Chiara Verpelli", "Carlo Sala", "Juergen Bockmann", "Stefan Tenzer", "Tobias M Boeckers", "Michael J Schmeisser"], "journal": "Frontiers in molecular neuroscience", "year": 2017, "abstract": "Disruption of the human SHANK3 gene can cause several neuropsychiatric disease entities including Phelan-McDermid syndrome, autism spectrum disorder (ASD), and intellectual disability. Although, a wide array of neurobiological studies strongly supports a major role for SHANK3 in organizing the post-synaptic protein scaffold, the molecular processes at synapses of individuals harboring SHANK3 mutations are still far from being understood. In this study, we biochemically isolated the post-synaptic density (PSD) fraction from striatum and hippocampus of adult Shank3Δ11-/- mutant mice and performed ion-mobility enhanced data-independent label-free LC-MS/MS to obtain the corresponding PSD proteomes (Data are available via ProteomeXchange with identifier PXD005192). This unbiased approach to identify molecular disturbances at Shank3 mutant PSDs revealed hitherto unknown brain region specific alterations including a striatal decrease of several molecules encoded by ASD susceptibility genes such as the serine/threonine kinase Cdkl5 and the potassium channel KCa1.1. Being the first comprehensive analysis of brain region specific PSD proteomes from a Shank3 mutant line, our study provides crucial information on molecular alterations that could foster translational treatment studies for SHANK3 mutation-associated synaptopathies and possibly also ASD in general.", "claims": [{"id": "claim-method", "statement": "Statistical analysis was performed using a Bonferroni-corrected unpaired two-tailed t-test and a sample size of n = 5 independent biological replicates.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28261056", "locator": "sections[10] \"Proteomic Characterization of the Striatal and Hippocampal PSD from Shank3Δ11-/- Mutants\" paragraph 3", "quote": "Statistical analysis was performed using a Bonferroni-corrected unpaired two-tailed t-test and a sample size of n = 5 independent biological replicates."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium2 via the PRIDE partner repository (Vizcaino et al., 2014, 2016) with the dataset identifier PXD005192.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28261056", "locator": "sections[8] \"Raw Data Processing and Data Analysis\" paragraph 3", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium2 via the PRIDE partner repository (Vizcaino et al., 2014, 2016) with the dataset identifier PXD005192."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:28261056"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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The mass spectrometry proteomics data have been deposited to the ProteomeXcha", "le. FIGURE S2Further characterization of the proteomic dataset. (A) Dynamic range of detected PSD p", "obility enhanced data-independent label-free LC-MS/MS to obtain the corresponding PSD proteomes (D"], "order_basis": "paper_text_order:0", "id": "assay:1"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["tant mice. (A) Schematic illustration of the murine Shank3 gene (boxes represent respective exon", "ntaining UniProtKB/Swiss-Prot entries of the mouse reference proteome (UniProtKB release 2014_0"], "order_basis": "paper_text_order:1862", "id": "assay:2"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ull#supplementary-material FIGURE S1Complete Western blot membranes. Complete membranes of the Western", "rn blot membranes. Complete membranes of the Western blots presented in Figure 1: (A,B) Subcellular fra", "ic illustration of the Shank3f isoform (B,C) Western blot analysis of striatal (B) and hippocampal (C)"], "order_basis": "paper_text_order:5750", "id": "assay:3"}]}