RNA immunoprecipitation identifies novel targets of DAZL in human foetal ovary.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:28364521", "kind": "science-record", "revision": 8, "content_hash": "sha256:7053b8598a246f29ae8ef962a192b5c96f7e9ce933bd27be34d9ea8abe73296d", "created_at": "2026-11-01T15:40:25Z", "updated_at": "2026-11-01T22:42:56Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "RNA immunoprecipitation identifies novel targets of DAZL in human foetal ovary.", "doi": "10.1093/molehr/gax004", "pmid": "28364521", "authors": ["Roseanne Rosario", "Richard W P Smith", "Ian R Adams", "Richard A Anderson"], "journal": "Molecular human reproduction", "year": 2017, "abstract": "Study question: Can novel meiotic RNA targets of DAZL (deleted in azoospermia-like) be identified in the human foetal ovary?\nSummary answer: SYCP1 (synaptonemal complex protein-1), TEX11 (testis expressed 11) and SMC1B (structural maintenance of chromosomes 1B) are novel DAZL targets in the human foetal ovary, thus DAZL may have previously unrecognised roles in the translational regulation of RNAs involved in chromosome cohesion and DNA recombination in the oocyte from the time of initiation of meiosis.\nWhat is known already: The phenotype of Dazl deficiency in mouse is infertility in both sexes and DAZL has also been linked to infertility in humans. Few studies have explored targets of this RNA-binding protein. The majority of these investigations have been carried out in mouse, and have focussed on the male thus the basis for its central function in regulating female fertility is largely unknown.\nStudy design size, duration: We carried out RNA sequencing after immunoprecipitation of endogenous DAZL from human foetal ovarian tissue (17 weeks of gestation, obtained after elective termination of pregnancy) to identify novel DAZL targets involved in meiosis (n = 3 biological replicates).\nParticipants/materials, setting, methods: Using quantitative RT-PCR, we examined the expression of selected RNAs identified by our immunoprecipitation across gestation, and visualised the expression of potential target SMC1B in relation to DAZL, with a combination of in situ hybridisation and immunohistochemistry. 3' untranslated region (3'UTR)-luciferase reporter assays and polysome profile analysis were used to investigate the regulation of three RNA targets by DAZL, representing key functionalities: SYCP1, TEX11 and SMC1B.\nMain results and the role of chance: We identified 764 potential RNA targets of DAZL in the human foetal ovary (false discovery rate 0.05 and log-fold change ≥ 2), with functions in synaptonemal complex formation (SYCP1, SYCP3), cohesin formation (SMC1B, RAD21), spindle assembly checkpoint (MAD2L1, TRIP13) and recombination and DNA repair (HORMAD1, TRIP13, TEX11, RAD18, RAD51). We demonstrated that the translation of novel targets SYCP1 (P = 0.004), TEX11 (P = 0.004) and SMC1B (P = 0.002) is stimulated by the presence of DAZL but not by a mutant DAZL with impaired RNA-binding activity.\nLarge scale data: The raw data are available at GEO using the study ID: GSE81524.\nLimitations, reasons for caution: This analysis is based on identification of DAZL targets at the time when meiosis starts in the ovary: it may have other targets at other stages of oocyte development, and in the testis. Representative targets were validated, but detailed analysis was not performed on the majority of putative targets.\nWider implications of the findings: These data indicate roles for DAZL in the regulation of several key functions in human oocytes. Through the translational regulation of novel RNA targets SMC1B and TEX11, DAZL may have a key role in regulating chromosome cohesion and DNA recombination; two processes fundamental in determining oocyte quality and whose establishment in foetal life may support lifelong fertility.\nStudy funding and competing interest(s): This study was supported by the UK Medical Research Council (grant no G1100357 to R.A.A. and an intramural MRC programme grant to I.R.A.). The authors declare no competing interests.", "claims": [{"id": "claim-method", "statement": "Differential analysis was performed using DESeq2 v1.14.0 (https://bioconductor.org/packages/release/bioc/html/DESeq2.html) using the biological replicate as a blocking factor in the design model to identify differences between control and DAZL immunoprecipitations.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28364521", "locator": "sections[5] \"RNA immunoprecipitation\" paragraph 2", "quote": "Differential analysis was performed using DESeq2 v1.14.0 (https://bioconductor.org/packages/release/bioc/html/DESeq2.html) using the biological replicate as a blocking factor in the design model to identify differences between control and DAZL immunoprecipitations."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The raw data are available at GEO using the study ID: GSE81524.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28364521", "locator": "sections[0] \"Abstract\" paragraph 7", "quote": "The raw data are available at GEO using the study ID: GSE81524."}], "state": "complete"}, {"id": "claim-target-result", "statement": "RNA sequencing after immunoprecipitation of endogenous DAZL identified 764 potential RNA targets in the human foetal ovary, and the raw data are available through GEO under study ID GSE81524.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:28364521", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "We carried out RNA sequencing after immunoprecipitation of endogenous DAZL from human foetal ovarian tissue (17 weeks of gestation, obtained after elective termination of pregnancy) to identify novel DAZL targets involved in meiosis (n = 3 biological replicates)."}, {"asset": "source:pmid:28364521", "locator": "sections[0] \"Abstract\" paragraph 6", "quote": "We identified 764 potential RNA targets of DAZL in the human foetal ovary (false discovery rate 0.05 and log-fold change ≥ 2), with functions in synaptonemal complex formation (SYCP1, SYCP3), cohesin formation (SMC1B, RAD21), spindle assembly checkpoint (MAD2L1, TRIP13) and recombination and DNA repair (HORMAD1, TRIP13, TEX11, RAD18, RAD51)."}, {"asset": "source:pmid:28364521", "locator": "sections[0] \"Abstract\" paragraph 7", "quote": "The raw data are available at GEO using the study ID: GSE81524."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "RNA sequencing after immunoprecipitation of endogenous DAZL identified 764 potential RNA targets in the human foetal ovary, and the raw data are available through GEO under study ID GSE81524.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:28364521"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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