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pmid:28404922
Adipophilin as prognostic biomarker in clear cell renal cell carcinoma.
Yuri Tolkach · Christine Lüders · Sebastian Meller · Klaus Jung · Carsten Stephan · Glen Kristiansen
Oncotarget · 2017 doi:10.18632/oncotarget.15639
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Complete file
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Immunohistochemistry with a polyclonal antibody to adipophilin was used on the tissue microarray (formalin-fixed, paraffin-embedded tissue) for detection of adipophilin. Median follow-up time was 91 (range 1-159) months in the whole cohort and 100 (1-159) months for patients with clear-cell RCC. Additional validation for adipophilin was performed using publicly available gene expression data for clear cell RCC from The Cancer Genome Atlas (TCGA).\nRESULTS: Adipophilin expression was detected in 14.3% of papillary RCC, in 0% of chromophobe RCC and in 58.7% of clear-cell RCC in the cytoplasm or at the membrane. Only membrane expression was correlated with other clinical parameters (pT-stage, pN-stage, R-status, sex) and showed a prognostic significance in univariate analysis with regard to overall survival of patients with clear cell subtype (HR 2.90, 95% CI 1.55-5.42, p=0.001), which failed significance on multivariate analysis. mRNA expression of PLIN2 on TCGA data using best selected cut-off was prognostically significant in both univariate (HR 1.76, 95% CI 1.28-2.42, p = 0.0005) and multivariate analyses (HR 1.46, 95% CI 1.05-2.04, p = 0.0257).\nCONCLUSIONS: Adipophilin is a novel and still understudied prognostic biomarker in clear cell renal cell carcinoma which deserves further study.", "claims": [{"id": "claim-method", "statement": "In multivariate Cox analysis with inclusion of pT-stage, pN-stage and WHO/ISUP-Grade of the tumor into the model the HR for low expression vs high expression was 1.46 (95% CI 1.05-2.04) with p = 0.0257 (see Supplementary Table 3).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28404922", "locator": "sections[6] \"Analysis of TCGA cohort\" paragraph 2", "quote": "In multivariate Cox analysis with inclusion of pT-stage, pN-stage and WHO/ISUP-Grade of the tumor into the model the HR for low expression vs high expression was 1.46 (95% CI 1.05-2.04) with p = 0.0257 (see Supplementary Table 3)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The extraction of the TCGA data (clinical data, mRNA expression data) was carried out for clear-cell subtype of the RCC (data version 28.01.2016).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28404922", "locator": "sections[15] \"Analysis of the TCGA data\" paragraph 2", "quote": "The extraction of the TCGA data (clinical data, mRNA expression data) was carried out for clear-cell subtype of the RCC (data version 28.01.2016)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:28404922"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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