Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:28419154", "kind": "science-record", "revision": 6, "content_hash": "sha256:245195a9e4de505e2a9e241a916d1917658130614179d45121181f7ebb2ffe80", "created_at": "2026-11-01T15:40:25Z", "updated_at": "2026-11-01T21:20:53Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots.", "doi": "10.1371/journal.pone.0176076", "pmid": "28419154", "authors": ["Lucas Z Passamani", "Roberta R Barbosa", "Ricardo S Reis", "Angelo S Heringer", "Patricia L Rangel", "Claudete Santa-Catarina", "Clícia Grativol", "Carlos F M Veiga", "Gonçalo A Souza-Filho", "Vanildo Silveira"], "journal": "PloS one", "year": 2017, "abstract": "Salt stress is one of the most common stresses in agricultural regions worldwide. In particular, sugarcane is affected by salt stress conditions, and no sugarcane cultivar presently show high productivity accompanied by a tolerance to salt stress. Proteomic analysis allows elucidation of the important pathways involved in responses to various abiotic stresses at the biochemical and molecular levels. Thus, this study aimed to analyse the proteomic effects of salt stress in micropropagated shoots of two sugarcane cultivars (CB38-22 and RB855536) using a label-free proteomic approach. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD006075. The RB855536 cultivar is more tolerant to salt stress than CB38-22. A quantitative label-free shotgun proteomic analysis identified 1172 non-redundant proteins, and 1160 of these were observed in both cultivars in the presence or absence of NaCl. Compared with CB38-22, the RB855536 cultivar showed a greater abundance of proteins involved in non-enzymatic antioxidant mechanisms, ion transport, and photosynthesis. Some proteins, such as calcium-dependent protein kinase, photosystem I, phospholipase D, and glyceraldehyde-3-phosphate dehydrogenase, were more abundant in the RB855536 cultivar under salt stress. Our results provide new insights into the response of sugarcane to salt stress, and the changes in the abundance of these proteins might be important for the acquisition of ionic and osmotic homeostasis during exposure to salt stress.", "claims": [{"id": "claim-method", "statement": "The same letter above the bars indicates no significant difference according to Tukey’s test at 5% probability.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28419154", "locator": "sections[10] \"Results\" paragraph 3", "quote": "The same letter above the bars indicates no significant difference according to Tukey’s test at 5% probability."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD006075.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28419154", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD006075."}], "state": "complete"}, {"id": "claim-target-result", "statement": "RB855536 exhibited greater salt tolerance than CB38-22; significance was assessed using Tukey’s test at 5% probability, and the mass-spectrometry proteomics source data are available via ProteomeXchange under identifier PXD006075.", "kind": "quantitative", "target": {"value": 5.0, "unit": "per cent", "tolerance": null, "source": "The same letter above the bars indicates no significant difference according to Tukey’s test at 5% probability."}, "evidence": [{"asset": "source:pmid:28419154", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The RB855536 cultivar is more tolerant to salt stress than CB38-22."}, {"asset": "source:pmid:28419154", "locator": "sections[10] \"Results\" paragraph 3", "quote": "The same letter above the bars indicates no significant difference according to Tukey’s test at 5% probability."}, {"asset": "source:pmid:28419154", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD006075."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "RB855536 exhibited greater salt tolerance than CB38-22; significance was assessed using Tukey’s test at 5% probability, and the mass-spectrometry proteomics source data are available via ProteomeXchange under identifier PXD006075.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:28419154"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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