Upregulation and biological function of transmembrane protein 119 in osteosarcoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:28496199", "kind": "science-record", "revision": 8, "content_hash": "sha256:cd10668f835f157c772a4bfc133a388856ce6fa07a2cf21a3eb6c8042a66e8fa", "created_at": "2026-11-01T15:40:25Z", "updated_at": "2026-11-01T21:59:22Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Upregulation and biological function of transmembrane protein 119 in osteosarcoma.", "doi": "10.1038/emm.2017.41", "pmid": "28496199", "authors": ["Zhen-Huan Jiang", "Jun Peng", "Hui-Lin Yang", "Xing-Li Fu", "Jin-Zhi Wang", "Lei Liu", "Jian-Nong Jiang", "Yong-Fei Tan", "Zhi-Jun Ge"], "journal": "Experimental & molecular medicine", "year": 2017, "abstract": "Osteosarcoma is suggested to be caused by genetic and molecular alterations that disrupt osteoblast differentiation. Recent studies have reported that transmembrane protein 119 (TMEM119) contributes to osteoblast differentiation and bone development. However, the level of TMEM119 expression and its roles in osteosarcoma have not yet been elucidated. In the present study, TMEM119 mRNA and protein expression was found to be up-regulated in osteosarcoma compared with normal bone cyst tissues. The level of TMEM119 protein expression was strongly associated with tumor size, clinical stage, distant metastasis and overall survival time. Moreover, gene set enrichment analysis (GSEA) of the Gene Expression Omnibus (GEO) GSE42352 dataset revealed TMEM119 expression in osteosarcoma tissues to be positively correlated with cell cycle, apoptosis, metastasis and TGF-β signaling. We then knocked down TMEM119 expression in U2OS and MG63 cells using small interfering RNA, which revealed that downregulation of TMEM119 could inhibit the proliferation of osteosarcoma cells by inducing cell cycle arrest in G0/G1 phase and apoptosis. We also found that TMEM119 knockdown significantly inhibited cell migration and invasion, and decreased the expression of TGF-β pathway-related factors (BMP2, BMP7 and TGF-β). TGF-β application rescued the inhibitory effects of TMEM119 knockdown on osteosarcoma cell migration and invasion. Further in vitro experiments with a TGF-β inhibitor (SB431542) or BMP inhibitor (dorsomorphin) suggested that TMEM119 significantly promotes cell migration and invasion, partly through TGF-β/BMP signaling. In conclusion, our data support the notion that TMEM119 contributes to the proliferation, migration and invasion of osteosarcoma cells, and functions as an oncogene in osteosarcoma.", "claims": [{"id": "claim-method", "statement": "To determine differentially expressed genes associated with TMEM119 expression, gene set enrichment analysis (GSEA) was performed based on the GSE42352 dataset using GSEA version 2.0 from the Broad Institute at MIT, as previously described.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28496199", "locator": "sections[15] \"Bioinformatic analysis\" paragraph 2", "quote": "To determine differentially expressed genes associated with TMEM119 expression, gene set enrichment analysis (GSEA) was performed based on the GSE42352 dataset using GSEA version 2.0 from the Broad Institute at MIT, as previously described."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Osteosarcoma gene expression data were obtained from Gene Expression Omnibus (GEO) using accession numbers GSE42352 and GSE39055. 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To determine differentially expressed genes associated with TMEM119 expression, gene set enrichment analysis (GSEA) was performed based on the GSE42352 dataset using GSEA version 2.0 from the Broad Institute at MIT, as previously described."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Using gene set enrichment analysis (GSEA) of the Gene Expression Omnibus (GEO) GSE42352 dataset, the paper found that TMEM119 expression in osteosarcoma tissues was positively correlated with cell cycle, apoptosis, metastasis, and TGF-β signaling.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:28496199", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Moreover, gene set enrichment analysis (GSEA) of the Gene Expression Omnibus (GEO) GSE42352 dataset revealed TMEM119 expression in osteosarcoma tissues to be positively correlated with cell cycle, apoptosis, metastasis and TGF-β signaling."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Using gene set enrichment analysis (GSEA) of the Gene Expression Omnibus (GEO) GSE42352 dataset, the paper found that TMEM119 expression in osteosarcoma tissues was positively correlated with cell cycle, apoptosis, metastasis, and TGF-β signaling.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:28496199"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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