Involvement of FAK-ERK2 signaling pathway in CKAP2-induced proliferation and motility in cervical carcinoma cell lines.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:28522860", "kind": "science-record", "revision": 7, "content_hash": "sha256:20e0060ebe67523d8871916670c03025e88b99ddf6a118d14da6269241f2d366", "created_at": "2026-11-01T15:40:26Z", "updated_at": "2026-11-01T21:20:55Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Involvement of FAK-ERK2 signaling pathway in CKAP2-induced proliferation and motility in cervical carcinoma cell lines.", "doi": "10.1038/s41598-017-01832-y", "pmid": "28522860", "authors": ["Qi-Sang Guo", "Yu Song", "Ke-Qin Hua", "Shu-Jun Gao"], "journal": "Scientific reports", "year": 2017, "abstract": "Cervical carcinoma is the fourth most common cause of death in woman, caused by human papillomavirus (HPV) infections and arising from the cervix. Cytoskeleton-associated protein 2 (CKAP2), also known as tumor-associated microtubule-associated protein, has been linked to tumorigenic effects. In the present study, we screened CKAP2 as a new candidate gene which promotes development of cervical carcinoma, in two independent datasets (TCGA and GSE27678). Results showed that CKAP2 expression was significantly up-regulated in cervical cancerous tissues compared with normal counterparts. Gene set enrichment analysis (GSEA) showed that metastasis, cell cycle and FAK pathways were related with elevated CKAP2 expression. Knockdown of CKAP2 expression significantly inhibited cell proliferation, migration and invasion both in HeLa and C-33A cells. And depletion of CKAP2 down-regulated the expression of metastasis and cell cycle related proteins as well as the phosphorylation of ERK2 (p-ERK2), except E-cadherin. In vivo experiment revealed that knockdown of CKAP2 inhibited C-33A cells proliferation. However, FAK inhibitor PF-562271 and ERK2 inhibitor VX-11e treatment significantly inhibited CKAP2 overexpression-induced cell proliferation, migration and invasion in SiHa cells. In conclusion, our study suggests that CKAP2 acts as a functional oncogene in cervical carcinoma development and may exert its function by targeting FAK-ERK2 signaling pathway.", "claims": [{"id": "claim-method", "statement": "To validate the correlation of CKAP2 and signaling pathway involved in pathogenesis in cervical carcinoma, a gene set enrichment analysis (GSEA) was performed to analyze the cervical carcinoma tumors in TCGA dataset.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28522860", "locator": "sections[13] \"Bioinformatics analysis\" paragraph 2", "quote": "To validate the correlation of CKAP2 and signaling pathway involved in pathogenesis in cervical carcinoma, a gene set enrichment analysis (GSEA) was performed to analyze the cervical carcinoma tumors in TCGA dataset."}], "state": "complete"}, {"id": "claim-data-access", "statement": "RNA-Seq data from cervical carcinoma tissues and adjacent tissues was downloaded from The Cancer Genome Atlas (TCGA) and the NCBIs Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/) are accessible through GEO Series accession number GSE27678 datasets following approval of this project by the consortium.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28522860", "locator": "sections[13] \"Bioinformatics analysis\" paragraph 2", "quote": "RNA-Seq data from cervical carcinoma tissues and adjacent tissues was downloaded from The Cancer Genome Atlas (TCGA) and the NCBIs Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/) are accessible through GEO Series accession number GSE27678 datasets following approval of this project by the consortium."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The paper used gene set enrichment analysis (GSEA) on CKAP2 expression in the TCGA and GSE27678 cervical carcinoma datasets; the GSEA identified metastasis-up, cell-cycle, and FAK pathways as associated with elevated CKAP2 expression.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:28522860", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "In the present study, we screened CKAP2 as a new candidate gene which promotes development of cervical carcinoma, in two independent datasets (TCGA and GSE27678)."}, {"asset": "source:pmid:28522860", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Gene set enrichment analysis (GSEA) showed that metastasis, cell cycle and FAK pathways were related with elevated CKAP2 expression."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The paper used gene set enrichment analysis (GSEA) on CKAP2 expression in the TCGA and GSE27678 cervical carcinoma datasets; the GSEA identified metastasis-up, cell-cycle, and FAK pathways as associated with elevated CKAP2 expression.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:28522860"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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All the cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Hy", "cervical carcinoma cell lines (C-33A, CaSki, HeLa, SiHa and C4-1) obtained from the Cell Bank", "9 and Snail) were significantly decreased in HeLa and C-33A cells infected with pLKO.1-EGFP-CK"], "order_basis": "paper_text_order:902", "id": "assay:2"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["ylation of ERK2 (p-ERK2), except E-cadherin. 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