Dataset to delineate changes in association between Akt1 and its interacting partners as a function of active state of Akt1 protein.

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Functional diversity of Akt1 is a result of its interactions with other proteins; which changes with changing context. This investigation was designed to capture the dynamics of Akt1 Interactome as a function of its active state. Delineating dynamic changes in association of Akt1 with its interactors could help us comprehend how it changes as a function of inhibition of its active form. Similar information on changes in Akt1 interactome as of now is not well explored. Akt1 expressing HEK293 cells were cultured in light and heavy labeled SILAC media. Normal lysine and arginine were incorporated as light labels while for heavy labeling the isotopes were 8 and 10 Da heavier. Light labeled cells represented the indigenous state of Akt1 interactome while heavy labeled cells represented Akt1 interactome in presence of its allosteric inhibitor, MK-2206. Equal number of cells from both conditions were pooled, lysed and subjected to Affinity Purification coupled to Mass Spectroscopy (AP-MS). Additionally, SILAC labeling aided in quantitative estimation of changing association of a number of proteins which were common to the two experimental conditions, with Akt1. Data are available via ProteomeXchange with identifier PXD005976.", "claims": [{"id": "claim-method", "statement": "Threshold of 1% accepted global false discovery rate from fit (G-FDR-fit) proteins and peptide spectrum matches (PSMs)", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28603765", "locator": "sections[11] \"Data processing in protein pilot\" paragraph 2", "quote": "Threshold of 1% accepted global false discovery rate from fit (G-FDR-fit) proteins and peptide spectrum matches (PSMs)"}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data is publicly available via ProteomeXchange with identifier ProteomeXchange: PXD005976.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28603765", "locator": "sections[3] \"Data\" paragraph 2", "quote": "Data is publicly available via ProteomeXchange with identifier ProteomeXchange: PXD005976."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study used SILAC labeling with AP-MS to compare Akt1 interactors in the indigenous state and after MK-2206 inhibition, with the source data deposited in ProteomeXchange under identifier PXD005976.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:28603765", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Akt1 expressing HEK293 cells were cultured in light and heavy labeled SILAC media."}, {"asset": "source:pmid:28603765", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Light labeled cells represented the indigenous state of Akt1 interactome while heavy labeled cells represented Akt1 interactome in presence of its allosteric inhibitor, MK-2206."}, {"asset": "source:pmid:28603765", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Data are available via ProteomeXchange with identifier PXD005976."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study used SILAC labeling with AP-MS to compare Akt1 interactors in the indigenous state and after MK-2206 inhibition, with the source data deposited in ProteomeXchange under identifier PXD005976.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:28603765"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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