← All .science files
pmid:28649586
Data from salivary gland proteome analysis of female Aedes aegypti Linn.
Rakhi Dhawan · Ajeet Kumar Mohanty · Manish Kumar · Gourav Dey · Jayshree Advani · T S Keshava Prasad · Ashwani Kumar
Data in brief · 2017 doi:10.1016/j.dib.2017.05.034
Paper
complete
Experiment
complete
Data
partial
Analysis
absent
Execution
absent
Execution evidence has not yet been added to this .science file. Explore its methods, data and open questions to plan the next step.
Record details sci_version · id · kind · revision · content_hash · schema · created_at · updated_at · generator
paper object · 10 fields
authors array · 7 items
claims array · 2 items
[0] object · 6 fields
evidence array · 1 item
[0] object · 3 fields
[1] object · 6 fields
target object · 4 fields
evidence array · 3 items
[0] object · 3 fields
[1] object · 3 fields
gaps array · 0 items
experiment object · 8 fields
steps array · 3 items
[0] object · 13 fields
parameters object · 0 fields
inputs array · 1 item
outputs array · 1 item
instruments array · 0 items
reagents array · 0 items
[1] object · 13 fields
parameters object · 0 fields
inputs array · 1 item
outputs array · 1 item
instruments array · 0 items
reagents array · 0 items
[2] object · 13 fields
parameters object · 0 fields
inputs array · 1 item
outputs array · 1 item
instruments array · 0 items
reagents array · 0 items
materials array · 0 items
instruments array · 0 items
reagents array · 0 items
conditions object · 0 fields
gaps array · 0 items
data object · 3 fields
assets array · 4 items
[0] object · 15 fields
structure object · 4 fields
[1] object · 15 fields
structure object · 15 fields
sample_accessions array · 0 items
[2] object · 16 fields
structure object · 5 fields
table object · 8 fields
rows array · 11 items
[0] array · 2 items
[1] array · 2 items
[2] array · 2 items
[3] array · 2 items
[4] array · 1 item
[5] array · 1 item
[6] array · 2 items
[7] array · 2 items
[8] array · 2 items
[9] array · 2 items
[10] array · 2 items
[3] object · 16 fields
structure object · 5 fields
table object · 8 fields
rows array · 11 items
[0] array · 2 items
[1] array · 2 items
[2] array · 2 items
[3] array · 2 items
[4] array · 1 item
[5] array · 1 item
[6] array · 2 items
[7] array · 2 items
[8] array · 2 items
[9] array · 2 items
[10] array · 2 items
gaps array · 2 items
capability object · 3 fields
requirements array · 1 item
[0] object · 6 fields
assay_families array · 9 items
gaps array · 0 items
readiness object · 8 fields
axes object · 3 fields
method object · 5 fields
required_operations array · 0 items
missing_operations array · 0 items
capability object · 4 fields
data object · 6 fields
accessions array · 1 item
resolved_accessions array · 1 item
statements array · 1 item
can_run object · 3 fields
dsl_operations array · 0 items
assets array · 4 items
steps array · 1 item
must_build object · 5 fields
dsl_operations array · 0 items
converters array · 0 items
readers array · 0 items
capabilities array · 0 items
data_gaps array · 1 item
gaps array · 1 item
review object · 5 fields
machine_critique object · 5 fields
reader object · 5 fields
evidence_check object · 2 fields
usage object · 3 fields
stages array · 1 item
[0] object · 4 fields
gaps array · 3 items
provenance object · 7 fields
entities array · 3 items
activities array · 3 items
[0] object · 4 fields
used array · 3 items
[0] object · 3 fields
[1] object · 3 fields
[2] object · 3 fields
[1] object · 4 fields
agents array · 2 items
lineage object · 4 fields
gaps array · 8 items
assays array · 1 item
[0] object · 11 fields
required_operations array · 0 items
source_hashes object · 1 field
evidence array · 4 items
Complete file
{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:28649586", "kind": "science-record", "revision": 5, "content_hash": "sha256:2f2e73feb8d8d421b882ac370fd380bfc8aa9c47798895b43948e74ecb5b20a5", "created_at": "2026-11-01T15:40:26Z", "updated_at": "2026-11-01T18:48:48Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Data from salivary gland proteome analysis of female Aedes aegypti Linn.", "doi": "10.1016/j.dib.2017.05.034", "pmid": "28649586", "authors": ["Rakhi Dhawan", "Ajeet Kumar Mohanty", "Manish Kumar", "Gourav Dey", "Jayshree Advani", "T S Keshava Prasad", "Ashwani Kumar"], "journal": "Data in brief", "year": 2017, "abstract": "Salivary gland proteins from female Aedes aegypti mosquito were extracted and analyzed on high-resolution mass spectrometry. Proteomic data was analysed using two search algorithms SEQUEST and Mascot, which results in acquisition of 83,836 spectra which were assigned to 5417 peptides belonging to 1208 proteins.These proteins were then assigned molecular functions and further analysis revealed biological processes they are involved in using Gene Ontology annotations. Several immunity related pathways were found to be enriched in salivary gland.The data of this study are also related to the research article \"Mosquito-Borne Diseases and Omics: Salivary gland proteome of the female Aedes aegypti mosquito\" (Dhawan et al., 2017) [1]. These data are deposited in ProteomeXchange in the public dataset PXD002468. In addition,a scientific interpretation of this dataset by Dhawan et al. [1] is available at http://dx.doi.org/10.1089/journal.omi.2016.0160.", "claims": [{"id": "claim-data-access", "statement": "Data accessibilityData are available here and via a web application (ProteomeXchange) Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD002468.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28649586", "locator": "sections[1] \"p\" paragraph 1", "quote": "Data accessibilityData are available here and via a web application (ProteomeXchange) Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD002468."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Using SEQUEST and Mascot with a 1% false discovery rate, the study assigned 83,836 spectra to 5,417 peptides belonging to 1,208 proteins; the source data are deposited in ProteomeXchange under PXD002468.", "kind": "quantitative", "target": {"value": 1.0, "unit": "per cent", "tolerance": null, "source": "A false discovery rate (FDR) of 1% was applied while identifying the peptide-spectrum matches (PSMs)."}, "evidence": [{"asset": "source:pmid:28649586", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Proteomic data was analysed using two search algorithms SEQUEST and Mascot, which results in acquisition of 83,836 spectra which were assigned to 5417 peptides belonging to 1208 proteins."}, {"asset": "source:pmid:28649586", "locator": "sections[8] \"Data analysis\" paragraph 2", "quote": "A false discovery rate (FDR) of 1% was applied while identifying the peptide-spectrum matches (PSMs)."}, {"asset": "source:pmid:28649586", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "These data are deposited in ProteomeXchange in the public dataset PXD002468."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Using SEQUEST and Mascot with a 1% false discovery rate, the study assigned 83,836 spectra to 5,417 peptides belonging to 1,208 proteins; the source data are deposited in ProteomeXchange under PXD002468.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:28649586"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD002468"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD002468"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:28649586", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1016/j.dib.2017.05.034", "sha256": "sha256:4c83c4a4345e729cd08ab6cf824d697b266a4c2edb87deb7cf0b0d7554f6d5f6", "bytes": 8779, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1016/j.dib.2017.05.034", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC5470437", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:PXD002468", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=PXD002468", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "PXD002468", "version": null, "note": "Data accessibilityData are available here and via a web application (ProteomeXchange) Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD002468.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Data accessibilityData are available here and via a web application (ProteomeXchange) Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD002468.", "access_statement_kind": "data_availability_statement", "sample_accessions": [], "sample_count": null, "group_sizes": null, "group_sizes_basis": null, "platform": null, "taxon": null, "title": "Proteomic analysis of salivary glands of female Aedes aegypti Linn", "resolution_checked_at": "2026-10-31T18:35:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "asset:table:pmid:28649586:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:38aaec1ae0456fa5c9246e96ea5cd311a0aad749239050028ff9d8f6b858d4fb", "bytes": 1100, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[1]/table-wrap[1]#t0005", "version": null, "note": null, "structure": {"pmcid": "PMC5470437", "section_index": 1, "section_title": "p", "row_count": 11, "column_count": 2}, "table": {"label": null, "caption": null, "rows": [["Subject area", "Biology"], ["More specific subject area", "Mosquito proteomics"], ["Type of data", "Mass spectrometry raw files, Excel tables, Graph, Figure"], ["How data was acquired", "LTQ-OrbitrapVelos ETD mass spectrometer (Thermo Scientific, Bremen, Germany)"], ["Proteome Discoverer 1.4 and MASCOT search engine (Matrix Science, London, UK; version 2.2)"], ["Protein database Aedes aegypti (www.VectorBase.org, release date21 Oct 2014)"], ["Data format", "Analyzed output data"], ["Experimental factors", "Salivary gland tissues were obtained from the female Aedes aegypti mosquitoes"], ["Experimental features", "Qualitative protein analysis of salivary gland tissue of Aedes aegypti"], ["Data source location", "Goa and Bangalore, India"], ["Data accessibility", "Data are available here and via a web application (ProteomeXchange) Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD002468."]], "row_count": 11, "column_count": 2, "numeric_cells": 3, "header_rows": 0, "locator": "section[1]/table-wrap[1]#t0005"}}, {"id": "asset:table:pmid:28649586:2", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:38aaec1ae0456fa5c9246e96ea5cd311a0aad749239050028ff9d8f6b858d4fb", "bytes": 1100, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[1]/table-wrap[1]#t0005", "version": null, "note": null, "structure": {"pmcid": "PMC5470437", "section_index": 2, "section_title": "p", "row_count": 11, "column_count": 2}, "table": {"label": null, "caption": null, "rows": [["Subject area", "Biology"], ["More specific subject area", "Mosquito proteomics"], ["Type of data", "Mass spectrometry raw files, Excel tables, Graph, Figure"], ["How data was acquired", "LTQ-OrbitrapVelos ETD mass spectrometer (Thermo Scientific, Bremen, Germany)"], ["Proteome Discoverer 1.4 and MASCOT search engine (Matrix Science, London, UK; version 2.2)"], ["Protein database Aedes aegypti (www.VectorBase.org, release date21 Oct 2014)"], ["Data format", "Analyzed output data"], ["Experimental factors", "Salivary gland tissues were obtained from the female Aedes aegypti mosquitoes"], ["Experimental features", "Qualitative protein analysis of salivary gland tissue of Aedes aegypti"], ["Data source location", "Goa and Bangalore, India"], ["Data accessibility", "Data are available here and via a web application (ProteomeXchange) Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD002468."]], "row_count": 11, "column_count": 2, "numeric_cells": 3, "header_rows": 0, "locator": "section[1]/table-wrap[1]#t0005"}}], "gaps": ["asset:data:geo:PXD002468 not downloaded", "asset:data:geo:PXD002468 licence not read"]}, "analysis": {"state": "absent", "methods": [], "targets": [{"id": "target-published-estimate", "claim_id": "claim-target-result", "operation": null, "expected_value": 1.0, "unit": "per cent", "tolerance": null, "state": "complete", "note": "The published estimate as the reader quoted it from the paper. No tolerance is published because the paper states none."}], "gaps": ["method", "operations"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "blocked", "tier": "R4", "blocking_axis": "method", "blocked_on": "method:not_determinable", "axes": {"method": {"tier": "R4", "expressible": false, "required_operations": [], "missing_operations": [], "basis": "The reader quoted the method the paper names (not named), but the corpus's method extractor could not map it onto a DSL operation, so the method is published but not expressible."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["PXD002468"], "resolved_accessions": ["PXD002468"], "statements": ["Data accessibilityData are available here and via a web application (ProteomeXchange) Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD002468."], "basis": "The paper names PXD002468 in ProteomeXchange and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": [], "assets": ["source:pmid:28649586", "data:geo:PXD002468", "asset:table:pmid:28649586:1", "asset:table:pmid:28649586:2"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:PXD002468"]}, "gaps": ["method:not_determinable"]}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-10-31T18:31:24Z", "independence": "same_provider"}, "evidence_check": {"method": null, "availability": "verbatim"}, "calls": 8, "usage": {"prompt_tokens": 20492, "completion_tokens": 2647, "cached_tokens": 1288}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:28649586", "type": "paper", "label": "pmid:28649586"}, {"id": "source:pmid:28649586", "type": "manuscript", "label": "PMID 28649586 full text"}, {"id": "data:geo:PXD002468", "type": "dataset", "label": "GEO PXD002468"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:28649586", "type": "paper", "label": "pmid:28649586"}, {"id": "source:pmid:28649586", "type": "manuscript", "label": "PMID 28649586 full text"}, {"id": "data:geo:PXD002468", "type": "dataset", "label": "GEO PXD002468"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-28649586", "type": "extraction", "used": ["source:pmid:28649586"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-PXD002468", "type": "accession_resolution", "used": ["data:geo:PXD002468"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:28649586", "parent_hash": "sha256:857e1212727ed4743f1a947f64cfd66f099533705e7bfdfaee50c7863ac0dee7", "fork_of": null, "generation": 4}, "gaps": ["asset:data:geo:PXD002468 licence not read", "asset:data:geo:PXD002468 not downloaded", "execution.not_run", "method", "operations", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "data_generation", "definition_id": "mass_spectrometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["re extracted and analyzed on high-resolution mass spectrometry. Proteomic data was analysed using two searc", "Tables S1 List of 1208 proteins derived from LC-MS/MS analysis of 18 fractions of Aedes aegypti sa", "Tables S2 List of 5417 peptides derived from LC-MS/MS analysis of 18 fractions of Aedes aegypti sa", "alyzed on high-resolution mass spectrometry. Proteomic data was analysed using two search algorithm"], "order_basis": "paper_text_order:134", "id": "assay:1"}]}