[Screening of biomarkers related with leukocyte responses early after burn injury in mice by differential gene expression profiling].

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:28669950", "kind": "science-record", "revision": 4, "content_hash": "sha256:e0e0816714477b62c24078cc571bba457d4219aa4e95e705b53b933b21560e2d", "created_at": "2026-11-01T15:40:27Z", "updated_at": "2026-11-01T21:59:25Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "partial", "title": "[Screening of biomarkers related with leukocyte responses early after burn injury in mice by differential gene expression profiling].", "doi": null, "pmid": "28669950", "authors": ["Qiong Zou", "Yan-Bin Gao", "Hui Jin", "Zhi-Yang Lu", "Peng-Wei Shi", "Lei Yang"], "journal": "Nan fang yi ke da xue xue bao = Journal of Southern Medical University", "year": 2017, "abstract": "OBJECTIVE: To screen the genes related with leukocyte responses in mice early after burn injury by bioinformatic analysis of the gene expression profiling data.\nMETHODS: The gene expression profiles were obtained from GEO (GSE7404, Mouse musculus, 25% TBSA, full-thickness) database. T test, fold changes and GO functional enrichment analysis were used to identify the differentially expressed genes (DEGs) related to leukocyte responses to burns; the interacting genes were transferred to STRING to construct the protein-protein interaction (PPI) network. Biological annotation of the sub-networks was executed using the software Cytoscape. Real-time PCR and Western blotting were used to verify the DEGs in mice.\nRESULTS: In mice at 1 day post-burn, a total of 658 genes were up-regulated and 1167 were down-regulated. PPI network and module analysis suggested that some of the genes (Stat1, Cdk1, Cd19, Lck and Jun) may play critical roles in the PPI network post-burn. Real-time PCR and Western blotting results in mice were consistent with those of bioinformatic analysis of Stat1, Cdk1 and Jun.\nCONCLUSION: Stat1, Cdk1 and Jun might be critical players in the development of leukocyte response in mice early after burn injury. 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