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pmid:28808310
Genome-scale analysis identifies NEK2, DLGAP5 and ECT2 as promising diagnostic and prognostic biomarkers in human lung cancer.
Yuan-Xiang Shi · Ji-Ye Yin · Yao Shen · Wei Zhang · Hong-Hao Zhou · Zhao-Qian Liu
Scientific reports · 2017 doi:10.1038/s41598-017-08615-5
Paper
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Experiment
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Data
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Analysis
partial
Execution
absent
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Complete file
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Genome-wide mRNA expression data obtained from the Gene Expression Omnibus (GSE19188, GSE18842 and GSE40791), including 231 primary tumor samples and 210 normal samples, were used to discover differentially expressed genes (DEGs). NEK2, DLGAP5 and ECT2 were found to be highly expressed in tumor samples. These results were experimentally confirmed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The elevated expression of the three candidate genes was also validated using the Cancer Genome Atlas (TCGA) datasets, which consist of 349 tumor and 58 normal tissues. Furthermore, we performed receiver operating characteristics (ROC) analysis to assess the diagnostic value of these lung cancer biomarkers, and the results suggested that NEK2, DLGAP5 and ECT2 expression levels could robustly distinguish lung cancer patients from normal subjects. 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subjects.", "rows": [["", "Lung cancer datasets", "AUC value", "P value", "95% CI", "Cut off value", "Specificity, sensitivity"], ["NEK2", "GSE19188", "0.9272", "<0.0001", "0.8826–0.9719", "−0.718", "0.890, 0.923"], ["GSE18842", "1", "<0.0001", "1.0000–1.0000", "4.384", "1.000, 1.000"], ["GSE40791", "0.9665", "<0.0001", "0.9415–0.9915", "3.991", "0.926, 0.910"], ["TCGA", "0.9733", "<0.0001", "0.9647–0.9883", "−0.871", "0.868, 0.983"], ["DLGAP5", "GSE19188", "0.9158", "<0.0001", "0.8703–0.9614", "−0.401", "0.846, 0.892"], ["GSE18842", "1", "<0.0001", "1.0000–1.0000", "4.505", "1.000, 1.000"], ["GSE40791", "0.9572", "<0.0001", "0.9297–0.9847", "4.043", "0.883, 0.940"], ["TCGA", "0.973", "<0.0001", "0.9578–0.9881", "−1.338", "0.905, 0.983"], ["ECT2", "GSE19188", "0.9013", "<0.0001", "0.8495–0.9530", "−0.008", "0.802, 0.969"], ["GSE18842", "0.9986", "<0.0001", "0.9950–1.002", "6.439", "0.978, 1.000"], ["GSE40791", "0.9728", "<0.0001", "0.9488–0.9967", "8.008", "0.926, 0.950"], ["TCGA", 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