miRNA Expression Profile and Effect of Wenxin Granule in Rats with Ligation-Induced Myocardial Infarction.

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However, the genomic pathological mechanisms of MI and mechanisms of WXKL are largely unknown. This study aims to investigate a comprehensive miRNA expression profile, and the predicted correlation pathways to be targeted by differentially expressed miRNAs in MI, and mechanisms of WXKL from a gene level. MI rat model was established by a coronary artery ligation surgery. miRNA expression microarrays were performed and the data were deposited in Gene Expression Omnibus (GEO number GSE95855). And, pathway analysis was performed by using the DIANA-miRPath v3.0 online tool. The expressions of miR-1, miR-133, Cx43, and Cx45 were detected by quantitative real-time PCR. It was found that 35 differentially expressed miRNAs and 23 predicted pathways, including miR-1, miR-133, and gap junction pathway, are involved in the pathogenesis of MI. And, WXKL increased the expressions of miR-1 and miR-133, while also increased the mRNA levels of Cx43 and Cx45, and, especially, recovered the Cx43/Cx45 ratio near to normal level. The results suggest that regulatory effects on miR-1, miR-133, Cx43, and Cx45 might be a possible mechanism of WXKL in the treatment of MI at the gene level.", "claims": [{"id": "claim-method", "statement": "Differentially expressed miRNAs were then identified through fold change as well as P value calculated using t-test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28894747", "locator": "sections[9] \"2.7. Bioinformatic Analysis\" paragraph 2", "quote": "Differentially expressed miRNAs were then identified through fold change as well as P value calculated using t-test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The microarray data discussed in this study have been deposited in the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) and are accessible through (GEO) Series accession number GSE95855 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?&acc=GSE95855).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28894747", "locator": "sections[8] \"2.6. 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MI rat model was established by a coronary artery l", "imals A total of 50 male Sprague-Dawley (SD) rats, weighted 200 ± 20 g, were acquired from Bei"], "order_basis": "paper_text_order:9", "id": "assay:1"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["Cx43, and Cx45 were detected by quantitative real-time PCR. It was found that 35 differentially express"], "order_basis": "paper_text_order:841", "id": "assay:2"}, {"role": "data_generation", "definition_id": "microarray", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["op of Figure 2). Heatmap of miRNA expression microarray from left ventricular tissue samples of MI r", "NAs between MI and non-MI rats identified by microarray. The miRNA clustering tree is hierarchically", "NAs between MI and non-MI rats identified by microarray. There were 23 predicted pathways integratin"], "order_basis": "paper_text_order:2753", "id": "assay:3"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 0.8, "evidence": ["analysis of variance"], "order_basis": "consumes_earlier_output", "id": "assay:4"}]}