A novel lncRNA, LL22NC03-N64E9.1, represses KLF2 transcription through binding with EZH2 in colorectal cancer.

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In the present study, we investigated lncRNAs alteration that contributed to colorectal cancer (CRC) by utilizing TCGA RNA sequencing data and other publicly available lncRNAs expression profiling data. Here, We screened out the CRC-associated lncRNA LL22NC03-N64E9.1, a key regulator of CRC development and progression. We also revealed that knockdown of LL22NC03-N64E9.1 inhibited cell proliferation, colony formation, tumorigenicity and apoptosis promotion, both in vitro and in vivo. Mechanistically, LL22NC03-N64E9.1 repressed underlying target gene KLF2 transcription through binding to EZH2. Furthermore, rescue experiments revealed that LL22NC03-N64E9.1 oncogenic function may partially depend on repressing KLF2. Taken together, our results suggested that LL22NC03-N64E9.1 confered an oncogenic function in human CRC and may serve as a candidate prognostic biomarker and target for new therapies in this deadly disease.", "claims": [{"id": "claim-method", "statement": "A paired, two-tailed Student's t-test or a chi-square test was used to evaluate significant differences between groups of data.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28938648", "locator": "sections[23] \"Statistical analysis\" paragraph 2", "quote": "A paired, two-tailed Student's t-test or a chi-square test was used to evaluate significant differences between groups of data."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The BAM files and normalized probe-level intensity files were downloaded from TCGA and GEO databases, respectively.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:28938648", "locator": "sections[10] \"Expression profiling data retrieval and analysis of lncRNAs in colorectal cancer\" paragraph 2", "quote": "The BAM files and normalized probe-level intensity files were downloaded from TCGA and GEO databases, respectively."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:28938648"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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