Quantitative proteomic Analysis Reveals up-regulation of caveolin-1 in FOXP3-overexpressed human gastric cancer cells.

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We have recently reported that FOXP3 inhibited proliferation of gastric cancer (GC) cells through activating the apoptotic signaling pathway. In this study, we found that over-expression of FOXP3 inhibited GC cell migration, invasion and proliferation. Then, the label-free quantitative proteomic approach was employed to further investigating the down-stream proteins regulated by FOXP3, resulting in a total of 3,978 proteins quantified, including 186 significantly changed proteins. Caveolin-1 (CAV1), as a main constituent protein of caveolae, was one of those changed proteins up-regulated in FOXP3-overexpressed GC cells, moreover, it was assigned as one of the node proteins in the protein-protein interaction network and the key protein involved in focal adhesion pathway by bioinformatics analysis. Further biological experiments confirmed that FOXP3 directly bound to the promoter regions of CAV1 to positively regulate CAV1 transcription in GC cells. In summary, our study suggested that FOXP3 can be considered as a tumor suppressor in GC via positively regulating CAV1 through transcriptional activation, and this FOXP3-CAV1 transcriptional regulation axis may play an important role in inhibiting invasion and metastasis of GC cells. Data are available via ProteomeXchange under identifier PXD007725.", "claims": [{"id": "claim-method", "statement": "Then the LFQ intensities were logarithmized, imputed with random numbers from a normal distribution (width = 0.3, shift = 1.8), and sent to Student’s t-test analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29089565", "locator": "sections[7] \"Data analysis\" paragraph 2", "quote": "Then the LFQ intensities were logarithmized, imputed with random numbers from a normal distribution (width = 0.3, shift = 1.8), and sent to Student’s t-test analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data are available via ProteomeXchange under identifier PXD007725.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29089565", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Data are available via ProteomeXchange under identifier PXD007725."}], "state": "complete"}, {"id": "claim-target-result", "statement": "FOXP3 overexpression upregulated CAV1 and inhibited gastric-cancer-cell migration, invasion, and proliferation; the label-free proteomic comparison identified 3,978 proteins and 186 significantly changed proteins using a fold change >1.5 and Students’ t test p value <0.01, with source data available via ProteomeXchange under identifier PXD007725.", "kind": "quantitative", "target": {"value": 0.01, "unit": "p-value", "tolerance": null, "source": "Then label-free proteomic experiments were performed to analyze the FOXP3-overexpressed AGS cells and vector cells, resulting in a total of 3978 proteins identified, of which 186 proteins were significantly changed (fold change >1.5, students’ t test p value <0.01) between these two types of cells."}, "evidence": [{"asset": "source:pmid:29089565", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "In summary, our study suggested that FOXP3 can be considered as a tumor suppressor in GC via positively regulating CAV1 through transcriptional activation, and this FOXP3-CAV1 transcriptional regulation axis may play an important role in inhibiting invasion and metastasis of GC cells."}, {"asset": "source:pmid:29089565", "locator": "sections[1] \"Introduction\" paragraph 6", "quote": "Then label-free proteomic experiments were performed to analyze the FOXP3-overexpressed AGS cells and vector cells, resulting in a total of 3978 proteins identified, of which 186 proteins were significantly changed (fold change >1.5, students’ t test p value <0.01) between these two types of cells."}, {"asset": "source:pmid:29089565", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Data are available via ProteomeXchange under identifier PXD007725."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "FOXP3 overexpression upregulated CAV1 and inhibited gastric-cancer-cell migration, invasion, and proliferation; the label-free proteomic comparison identified 3,978 proteins and 186 significantly changed proteins using a fold change >1.5 and Students’ t test p value <0.01, with source data available via ProteomeXchange under identifier PXD007725.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:29089565"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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