Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia.

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:29133510", "kind": "science-record", "revision": 2, "content_hash": "sha256:750f7ca371a26e65e2db9b208b2c9cd6444343223baac5a22bc1708248ce95d6", "created_at": "2026-11-01T15:40:29Z", "updated_at": "2026-11-01T18:49:06Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia.", "doi": "10.1074/mcp.RA117.000419", "pmid": "29133510", "authors": ["Teresa García-Berrocoso", "Víctor Llombart", "Laura Colàs-Campàs", "Alexandre Hainard", "Virginie Licker", "Anna Penalba", "Laura Ramiro", "Alba Simats", "Alejandro Bustamante", "Elena Martínez-Saez", "Francesc Canals", "Jean-Charles Sanchez", "Joan Montaner"], "journal": "Molecular & cellular proteomics : MCP", "year": 2018, "abstract": "Cerebral ischemia entails rapid tissue damage in the affected brain area causing devastating neurological dysfunction. How each component of the neurovascular unit contributes or responds to the ischemic insult in the context of the human brain has not been solved yet. Thus, the analysis of the proteome is a straightforward approach to unraveling these cell proteotypes. In this study, post-mortem brain slices from ischemic stroke patients were obtained corresponding to infarcted (IC) and contralateral (CL) areas. By means of laser microdissection, neurons and blood brain barrier structures (BBB) were isolated and analyzed using label-free quantification. MS data are available via ProteomeXchange with identifier PXD003519. Ninety proteins were identified only in neurons, 260 proteins only in the BBB and 261 proteins in both cell types. Bioinformatics analyses revealed that repair processes, mainly related to synaptic plasticity, are outlined in microdissected neurons, with nonexclusive important functions found in the BBB. A total of 30 proteins showing p < 0.05 and fold-change> 2 between IC and CL areas were considered meaningful in this study: 13 in neurons, 14 in the BBB and 3 in both cell types. Twelve of these proteins were selected as candidates and analyzed by immunohistofluorescence in independent brains. The MS findings were completely verified for neuronal SAHH2 and SRSF1 whereas the presence in both cell types of GABT and EAA2 was only validated in neurons. In addition, SAHH2 showed its potential as a prognostic biomarker of neurological improvement when analyzed early in the plasma of ischemic stroke patients. Therefore, the quantitative proteomes of neurons and the BBB (or proteotypes) after human brain ischemia presented here contribute to increasing the knowledge regarding the molecular mechanisms of ischemic stroke pathology and highlight new proteins that might represent putative biomarkers of brain ischemia or therapeutic targets.", "claims": [{"id": "claim-target-result", "statement": "Using label-free quantification, the study identified 30 proteins showing p < 0.05 and fold-change > 2 between infarcted and contralateral areas, with the MS source data available through ProteomeXchange under identifier PXD003519.", "kind": "quantitative", "target": {"value": 0.05, "unit": "p-value", "tolerance": null, "source": "A total of 30 proteins showing p < 0.05 and fold-change> 2 between IC and CL areas were considered meaningful in this study: 13 in neurons, 14 in the BBB and 3 in both cell types."}, "evidence": [{"asset": "source:pmid:29133510", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "By means of laser microdissection, neurons and blood brain barrier structures (BBB) were isolated and analyzed using label-free quantification."}, {"asset": "source:pmid:29133510", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "MS data are available via ProteomeXchange with identifier PXD003519."}, {"asset": "source:pmid:29133510", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "A total of 30 proteins showing p < 0.05 and fold-change> 2 between IC and CL areas were considered meaningful in this study: 13 in neurons, 14 in the BBB and 3 in both cell types."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Using label-free quantification, the study identified 30 proteins showing p < 0.05 and fold-change > 2 between infarcted and contralateral areas, with the MS source data available through ProteomeXchange under identifier PXD003519.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:29133510"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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