MAPK, NFκB, and VEGF signaling pathways regulate breast cancer liver metastasis.

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We constructed a genetic interaction network with 84 nodes and 237 edges using the String consortium database. The network was reliably robust with a clustering coefficient (cc) of 0.598 and protein-protein interaction (PPI) enrichment p value of zero. Using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases, we identified MAPK, NFκB and VEGF signaling pathways as the most critical pathways regulating breast cancer liver metastasis. These results indicate that the distinct breast cancer metastatic stages, including dissemination from the primary breast tumor, transit through the vasculature, and survival and proliferation in the liver, are regulated by the MAPK, NFκB, and VEGF signaling pathways.", "claims": [{"id": "claim-method", "statement": "Then, Significance Analysis of Microarrays software (SAM, http://statweb.stanford.edu/~tibs/SAM/) was used to produce a cluster of up- or down-regulated genes [27].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29254177", "locator": "sections[10] \"Analysis of differentially expressed genes\" paragraph 2", "quote": "Then, Significance Analysis of Microarrays software (SAM, http://statweb.stanford.edu/~tibs/SAM/) was used to produce a cluster of up- or down-regulated genes [27]."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Microarray dataset with the accession number GSE62598 was downloaded from Gene Expression Omnibus (GEO). 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