[Effects of low salt diet on gene expression in dog's heart].

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:29256234", "kind": "science-record", "revision": 4, "content_hash": "sha256:2142e65988c55f452dcbcea3e262df79d06c72778a01a6c8adc0dcbe1c28a190", "created_at": "2026-11-01T15:40:30Z", "updated_at": "2026-11-01T21:59:37Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "[Effects of low salt diet on gene expression in dog's heart].", "doi": "10.3785/j.issn.1008-9292.2017.08.14", "pmid": "29256234", "authors": ["Xiaomei Ren", "Bao Xin", "Wenwen Qian", "Rongqiang Zhang"], "journal": "Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences", "year": 2017, "abstract": "OBJECTIVE: To investigate the effect of low-salt diet on gene expression of canine cardiac tissue.\nMETHODS: Microarray data (GSE17149) of heart tissue from dogs fed with low-salt diet was obtained from the Gene Expression Omnibus (GEO). The data were analyzed by Qlucore Omics Explorer 3.1, STRING 10.0, Genclip 2.0 and GCBI. The protein-protein interactions (PPI) of the differentially expressed genes in low-salt and control groups were conducted to screen the key node genes between the two groups.\nRESULTS: Compared with the control group, the gene expression profile of the dog heart tissue was changed in the low-salt diet group, and 1343 (3.12%) differentially expressed genes were found in 43 035 genes. The differentially expressed gene protein interaction analysis revealed a most obvious protein (NFKBIA) as the core, which might be related to inhibiting the differentiation of macrophage-derived foam cell and promoting cholesterol discharge and decomposition.\nCONCLUSIONS: Gene expression of heart tissue in dogs fed with low-salt diet is significantly changed and low-salt diet may reduce the risk of cardiovascular disease by up-regulating the expression of NFKBIA.", "claims": [{"id": "claim-target-result", "statement": "与正常饮食组比较，低盐饮食组犬心脏组织基因表达谱发生改变，在43 035个基因中共有1343个（3.12%）差异表达基因；但摘要未说明具体统计方法，数据来源路线为从GEO公共数据库获取GSE17149心脏组织基因芯片数据。", "kind": "quantitative", "target": {"value": 3.12, "unit": "per cent", "tolerance": null, "source": "与正常饮食组比较，低盐饮食组犬心脏组织基因表达谱发生改变，在43 035个基因中共有1343个（3.12%）差异表达基因；差异表达基因蛋白质相互作用网络分析发现1个最明显的蛋白质互相作用网络，以NF-κB抑制因子-α（NFKBIA）蛋白为核心，主要发挥减弱巨噬细胞源性泡沫细胞分化、促进胆固醇排出和分解等生物学功能。"}, "evidence": [{"asset": "source:pmid:29256234", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "从基因芯片公共数据库（GEO）中获取低盐饮食心脏组织基因芯片数据（GSE17149），将数据导入Qlucore Omics Explorer 3.1、STRING 10.0、Genclip 2.0、GCBI等分析软件，筛选低盐饮食组与正常饮食组实验动物心脏组织差异表达基因，并分析差异表达基因编码蛋白质相互作用网络等，筛选两组之间的关键节点基因。"}, {"asset": "source:pmid:29256234", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "与正常饮食组比较，低盐饮食组犬心脏组织基因表达谱发生改变，在43 035个基因中共有1343个（3.12%）差异表达基因；差异表达基因蛋白质相互作用网络分析发现1个最明显的蛋白质互相作用网络，以NF-κB抑制因子-α（NFKBIA）蛋白为核心，主要发挥减弱巨噬细胞源性泡沫细胞分化、促进胆固醇排出和分解等生物学功能。"}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "与正常饮食组比较，低盐饮食组犬心脏组织基因表达谱发生改变，在43 035个基因中共有1343个（3.12%）差异表达基因；但摘要未说明具体统计方法，数据来源路线为从GEO公共数据库获取GSE17149心脏组织基因芯片数据。", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:29256234"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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