Increased mediator complex subunit 15 expression is associated with poor prognosis in hepatocellular carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:29556287", "kind": "science-record", "revision": 9, "content_hash": "sha256:c7086a83437fd0b65bc9c3b512c08499eb0fe632f5cf55d692719719051671d1", "created_at": "2026-11-01T15:40:32Z", "updated_at": "2026-11-01T22:43:19Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Increased mediator complex subunit 15 expression is associated with poor prognosis in hepatocellular carcinoma.", "doi": "10.3892/ol.2018.7820", "pmid": "29556287", "authors": ["Kunyuan Wang", "Chenxi Duan", "Xuejing Zou", "Yang Song", "Wenwen Li", "Lushan Xiao", "Jie Peng", "Liheng Yao", "Qian Long", "Li Liu"], "journal": "Oncology letters", "year": 2018, "abstract": "Mediator complex subunit 15 (MED15) is a coactivator involved in the regulated transcription of RNA polymerase II-dependent genes and serves an oncogenic role in numerous types of cancer. However, the expression and function of MED15 in hepatocellular carcinoma (HCC) remain unknown. In the present study, the aim was to investigate the expression and clinical significance of MED15 in HCC. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemical analysis revealed that MED15 mRNA and protein levels were significantly upregulated in HCC tissues compared with those in the corresponding adjacent non-tumor liver tissues. Furthermore, analyzing data from The Cancer Genome Atlas-Liver Hepatocellular Carcinoma (TCGA-LIHC) and GSE14520 datasets revealed a significant correlation between MED15 expression and the tumor size (P=0.033), Barcelona Clinic Liver Cancer stage (P=0.031), α-fetoprotein levels (P=0.002) and metastasis risk (P=0.001). Furthermore, patients with high MED15 expression levels had a shorter survival time compared with those with low MED15 expression levels (P<0.05). Univariate and multivariate analyses further revealed that MED15 may be an independent prognostic factor for the overall survival of HCC patients (hazard ratio, 1.762; 95% confidence interval, 1.077-2.882; P<0.05). In addition, MED15 expression was positively associated with hypoxia-inducible factor 1α expression in the TCGA-LIHC and GSE14520 datasets (P<0.01). In conclusion, the data reported in the present study indicated that MED15 is overexpressed in HCC and may represent a novel prognostic biomarker for patients with HCC.", "claims": [{"id": "claim-method", "statement": "Kaplan-Meier and log-rank test analyses were employed to test for an association between MED15 expression and the OS or DFS of the patients.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29556287", "locator": "sections[16] \"MED15 expression levels predict the clinical outcome of patients with HCC\" paragraph 2", "quote": "Kaplan-Meier and log-rank test analyses were employed to test for an association between MED15 expression and the OS or DFS of the patients."}], "state": "complete"}, {"id": "claim-data-access", "statement": "In order to investigate the role of MED15 expression in HCC, the current study analyzed the TCGA-LIHC dataset (http://www.cbioportal.org/data_sets.jsp) that contains 331 HCC cases and the GSE14520 dataset (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE14520) that provides 246 HCC cases (hereafter defined as the TCGA-LIHC and GSE14520 cohorts, respectively). These datasets comprise follow-up information for patients and gene expression data.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29556287", "locator": "sections[8] \"TCGA-LIHC and GSE14520 datasets, Oncomine gene expression array\" paragraph 2", "quote": "In order to investigate the role of MED15 expression in HCC, the current study analyzed the TCGA-LIHC dataset (http://www.cbioportal.org/data_sets.jsp) that contains 331 HCC cases and the GSE14520 dataset (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE14520) that provides 246 HCC cases (hereafter defined as the TCGA-LIHC and GSE14520 cohorts, respectively). These datasets comprise follow-up information for patients and gene expression data."}], "state": "complete"}, {"id": "claim-target-result", "statement": "MED15 mRNA and protein expression were significantly upregulated in HCC tissues, and high MED15 expression was associated with poor prognosis; the study used RT-qPCR and immunohistochemical analysis of tissue specimens and analyzed the TCGA-LIHC and GSE14520 datasets for clinicopathological and survival associations.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:29556287", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemical analysis revealed that MED15 mRNA and protein levels were significantly upregulated in HCC tissues compared with those in the corresponding adjacent non-tumor liver tissues."}, {"asset": "source:pmid:29556287", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Furthermore, analyzing data from The Cancer Genome Atlas-Liver Hepatocellular Carcinoma (TCGA-LIHC) and GSE14520 datasets revealed a significant correlation between MED15 expression and the tumor size (P=0.033), Barcelona Clinic Liver Cancer stage (P=0.031), α-fetoprotein levels (P=0.002) and metastasis risk (P=0.001)."}, {"asset": "source:pmid:29556287", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "In conclusion, the data reported in the present study indicated that MED15 is overexpressed in HCC and may represent a novel prognostic biomarker for patients with HCC."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "MED15 mRNA and protein expression were significantly upregulated in HCC tissues, and high MED15 expression was associated with poor prognosis; the study used RT-qPCR and immunohistochemical analysis of tissue specimens and analyzed the TCGA-LIHC and GSE14520 datasets for clinicopathological and survival associations.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:29556287"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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