Characterization of the adult Aedes aegypti early midgut peritrophic matrix proteome using LC-MS.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:29570734", "kind": "science-record", "revision": 4, "content_hash": "sha256:4c161faa02051efa2373270d2e0a008ce6cea62fcbb9b4cc4b56735b78ad511e", "created_at": "2026-11-01T15:40:32Z", "updated_at": "2026-11-01T21:21:21Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Characterization of the adult Aedes aegypti early midgut peritrophic matrix proteome using LC-MS.", "doi": "10.1371/journal.pone.0194734", "pmid": "29570734", "authors": ["Shavonn R Whiten", "W Keith Ray", "Richard F Helm", "Zach N Adelman"], "journal": "PloS one", "year": 2018, "abstract": "The Aedes aegypti mosquito is the principal vector of arboviruses such as dengue, chikungunya, yellow fever, and Zika virus. These arboviruses are transmitted during adult female mosquito bloodfeeding. While these viruses must transverse the midgut to replicate, the blood meal must also reach the midgut to be digested, absorbed, or excreted, as aggregation of blood meal metabolites can be toxic to the female mosquito midgut. The midgut peritrophic matrix (PM), a semipermeable extracellular layer comprised of chitin fibrils, glycoproteins, and proteoglycans, is one such mechanism of protection for the mosquito midgut. However, this structure has not been characterized for adult female Ae. aegypti. We conducted a mass spectrometry based proteomic analysis to identify proteins that comprise or are associated with the adult female Ae. aegypti early midgut PM. Altogether, 474 unique proteins were identified, with 115 predicted as secreted. GO-term enrichment analysis revealed an abundance of serine-type proteases and several known and novel intestinal mucins. In addition, approximately 10% of the peptides identified corresponded to known salivary proteins, indicating Ae. aegypti mosquitoes extensively swallow their own salivary secretions. However, the physiological relevance of this remains unclear, and further studies are needed to determine PM proteins integral for midgut protection from blood meal derived toxicity and pathogen protection. Finally, we describe substantial discordance between previously described transcriptionally changes observed in the midgut in response to a bloodmeal and the presence of the corresponding protein in the PM. Data are available via ProteomeXchange with identifier PXD007627.", "claims": [{"id": "claim-method", "statement": "g: GOSt—gene group functional profiling (version: r1709_e87_eg34) was used to conduct a hypergeometric enrichment analysis for the 115 secreted proteins isolated in our LC-MS based proteomic analysis with a predicted secretory signal peptide [31, 32]. The unordered list of VectorBase Gene Stable IDs was entered in the g: GOSt user interface.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29570734", "locator": "sections[6] \"Bioinformatic analyses\" paragraph 3", "quote": "g: GOSt—gene group functional profiling (version: r1709_e87_eg34) was used to conduct a hypergeometric enrichment analysis for the 115 secreted proteins isolated in our LC-MS based proteomic analysis with a predicted secretory signal peptide [31, 32]. The unordered list of VectorBase Gene Stable IDs was entered in the g: GOSt user interface."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data are available via ProteomeXchange with identifier PXD007627.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29570734", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Data are available via ProteomeXchange with identifier PXD007627."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study used LC-MS proteomics and computational resampling to estimate proteome saturation, identifying 474 unique early peritrophic-matrix proteins, including 115 predicted secreted proteins; the source proteomics data were deposited in ProteomeXchange under identifier PXD007627.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:29570734", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Altogether, 474 unique proteins were identified, with 115 predicted as secreted."}, {"asset": "source:pmid:29570734", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Data are available via ProteomeXchange with identifier PXD007627."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study used LC-MS proteomics and computational resampling to estimate proteome saturation, identifying 474 unique early peritrophic-matrix proteins, including 115 predicted secreted proteins; the source proteomics data were deposited in ProteomeXchange under identifier PXD007627.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:29570734"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD007627"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD007627"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:29570734", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1371/journal.pone.0194734", "sha256": "sha256:5defeaebcf7963876f167bfc572bc1f608aaa9e0f03f863b65479022223cd41e", "bytes": 52960, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1371/journal.pone.0194734", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC5865745", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:PXD007627", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=PXD007627", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "PXD007627", "version": null, "note": "Data are available via ProteomeXchange with identifier PXD007627.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Data are available via ProteomeXchange with identifier PXD007627.", "access_statement_kind": "data_availability_statement", "sample_accessions": [], "sample_count": null, "group_sizes": null, "group_sizes_basis": null, "platform": null, "taxon": null, "title": "The Adult Aedes aegypti Midgut Peritrophic Matrix Proteome", "resolution_checked_at": "2026-10-31T18:35:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "asset:table:pmid:29570734:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:f4725c9e2c6b4d5e97b9d2b039fa79fe8c92e3fb8a4658c909472567c273620e", "bytes": 828, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[12]/table-wrap[1]#pone.0194734.t001", "version": null, "note": "Known and putative adult Ae. aegypti peritrophic matrix proteins identified by LC-MS with predicted chitin-binding domains.The mass is based on the primary amino acid sequence and does not account for glycosylation.", "structure": {"pmcid": "PMC5865745", "section_index": 12, "section_title": "Chitin-binding proteins", "row_count": 5, "column_count": 4}, "table": {"label": "Table 1", "caption": "Known and putative adult Ae. aegypti peritrophic matrix proteins identified by LC-MS with predicted chitin-binding domains.The mass is based on the primary amino acid sequence and does not account for glycosylation.", "rows": [["Gene Stable ID a", "Gene Name", "Predicted MW b", "Annotation/Comments b,c,d"], ["AAEL002495", "AeIMUC1", "30.6", "3 CBD; Peritrophin-A domain; Mucin domain; O-glycosylated; SP; UF"], ["AAEL002467", "AeAper50", "54.2", "5 CBD; Peritrophin-A domain; N- and O-glycosylated; SP; UF"], ["AAEL006953", "-", "31.3", "2 CBD; Peritrophin-A domain; N-glycosylated; SP; UF"], ["AAEL004798", "-", "39.6", "3 CBD; Peritrophin-A domain; Mucin domain; O-glycosylated; SP; TM; UF"]], "row_count": 5, "column_count": 4, "numeric_cells": 13, "header_rows": 1, "locator": "section[12]/table-wrap[1]#pone.0194734.t001"}}, {"id": "asset:table:pmid:29570734:2", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:9b01a8a6c116417f1498f9e31fdee21b41376bedbaee19e144b559c6a92661fb", "bytes": 1070, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[13]/table-wrap[1]#pone.0194734.t002", "version": null, "note": "Adult female Ae. aegypti secreted proteins and one to one orthologs isolated in Dinglasan et al. [33] An. gambiae adult female LC-MS peritrophic matrix proteomic analysis.", "structure": {"pmcid": "PMC5865745", "section_index": 13, "section_title": "Orthologs to Anopheles gambiae PM proteomic analysis", "row_count": 11, "column_count": 4}, "table": {"label": "Table 2", "caption": "Adult female Ae. aegypti secreted proteins and one to one orthologs isolated in Dinglasan et al. 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We conducted a mass spectrometry based proteomic analysis to identify protein", "ypti. We conducted a mass spectrometry based proteomic analysis to identify proteins that comprise", "g low melting agarose to produce a rigid PM. Proteomic analysis was conducted (LC-MS). (B) Adult fe"], "order_basis": "paper_text_order:774", "id": "assay:1"}]}