Expression of the microRNA-143/145 cluster is decreased in hepatitis B virus-associated hepatocellular carcinoma and may serve as a biomarker for tumorigenesis in patients with chronic hepatitis B.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:29616093", "kind": "science-record", "revision": 8, "content_hash": "sha256:18a15b8bea05dab4fb15ebaa37be83acc6382e822550103bc4811dfbd908e975", "created_at": "2026-11-01T15:40:33Z", "updated_at": "2026-11-01T21:59:47Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Expression of the microRNA-143/145 cluster is decreased in hepatitis B virus-associated hepatocellular carcinoma and may serve as a biomarker for tumorigenesis in patients with chronic hepatitis B.", "doi": "10.3892/ol.2018.8117", "pmid": "29616093", "authors": ["Qi Zhao", "Xiangfei Sun", "Chao Liu", "Tao Li", "Juan Cui", "Chengyong Qin"], "journal": "Oncology letters", "year": 2018, "abstract": "The aims of the present study were to identify the expression profile of microRNA (miR)-143/145 in hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC), explore its association with prognosis and investigate whether the serum miR-143/145 expression levels may serve as a diagnostic indicator of HBV-associated HCC. The microRNA (miRNA) chromatin immunoprecipitation dataset was obtained from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus databases, and analyzed using the Wilcoxon signed-rank test. It was observed that the expression of miR-143 and miR-145 was decreased 1.5-fold in HBV-associated HCC samples compared with non-tumor tissue in the TCGA and the GSE22058 datasets (P<0.01). Using the reverse transcription-quantitative polymerase chain reaction, it was further confirmed that miR-143/145 and their host gene MIR143HG were downregulated in HBV-associated HCC tissues compared with corresponding distal non-tumor tissues. The lower level of miR-143 and miR-145 expression was associated with tumor differentiation, and may thus be responsible for a poor prognosis of patients with HBV-associated HCC. The receiver-operating characteristic (ROC) curves were used to explore the potential value of miR-143 and miR-145 as biomarkers for predicting HBV-associated HCC tumorigenesis. In serum, miR-143/145 were identified to be significantly decreased in patients with HBV-associated HCC compared with negative control patients, and their associated areas under the ROC curves were calculated at 0.813 and 0.852 (P<0.05), with each having a sensitivity and a specificity close to 0.80. These results indicated that the decreased expression of the miR-143/145 cluster and their host gene MIR143HG in HBV-associated HCC tissue was associated with prognosis, and each of these miRNAs may serve as a valuable diagnostic biomarker for predicting HBV-associated HCC tumorigenesis.", "claims": [{"id": "claim-method", "statement": "For each dataset, the Wilcoxon signed-rank test was used to identify the differentially expressed genes between tumor and non-tumor samples.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29616093", "locator": "sections[6] \"Identification of differentially expressed miRNAs\" paragraph 2", "quote": "For each dataset, the Wilcoxon signed-rank test was used to identify the differentially expressed genes between tumor and non-tumor samples."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The miRNA expression profile of 372 patients with HCC and 50 non-tumor distal liver samples was obtained from the TCGA dataset (cancergenome.nih.gov).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29616093", "locator": "sections[4] \"TCGA miRNA dataset\" paragraph 2", "quote": "The miRNA expression profile of 372 patients with HCC and 50 non-tumor distal liver samples was obtained from the TCGA dataset (cancergenome.nih.gov)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Using the Wilcoxon signed-rank test on miRNA ChIP data obtained from TCGA and GEO, miR-143 and miR-145 expression was decreased 1.5-fold in HBV-associated HCC samples compared with non-tumor tissue (P<0.01).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:29616093"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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