MiR-152 functioning as a tumor suppressor that interacts with DNMT1 in nasopharyngeal carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:29628766", "kind": "science-record", "revision": 8, "content_hash": "sha256:319d6d81f8ffe92b3f249fe9450a98b69a9b0b04d94916c12d1ee01698b443b0", "created_at": "2026-11-01T15:40:33Z", "updated_at": "2026-11-01T22:43:20Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "MiR-152 functioning as a tumor suppressor that interacts with DNMT1 in nasopharyngeal carcinoma.", "doi": "10.2147/OTT.S154464", "pmid": "29628766", "authors": ["Zhi-Wei Lu", "Ming-Yu Du", "Lu-Xi Qian", "Nan Zhang", "Jia-Jia Gu", "Kai Ding", "Jing Wu", "Hong-Ming Zhu", "Xia He", "Li Yin"], "journal": "OncoTargets and therapy", "year": 2018, "abstract": "BACKGROUND: In recent years, miR-152 has been dysregulated in a variety of tumors and used as a tumor suppressor. Nevertheless, its role in nasopharyngeal carcinoma (NPC) remains unidentified.\nMATERIALS AND METHODS: Real-time quantitative PCR (polymerase chain reaction) was performed to analyze the expression of miR-152 in NPC cell lines. MiR-152 expression profiles in NPC tissues were obtained from Gene Expression Omnibus (GEO GSE36682). The effect of miR-152 on the invasion and proliferation of NPC cells was determined through cell invasion, wound healing, and cell viability assays. Apoptosis was examined by flow cytometry, and Western blot was performed to measure expression of the target gene. Pyrosequencing was used to detect the methylation level of NPC cells.\nRESULTS: In this study, miR-152 was downregulated in the NPC tissues and cell lines. When miR-152 was enhanced, the invasion and migration of NPC cells were inhibited. However, miR-152 had no effect on the proliferation of NPC cells. Luciferase reporter gene analysis was performed, and the results showed that DNMT1 (DNA (cytosine-5)-methyltransferase 1) is a direct target of miR-152 in NPC cells. DNMT1 downregulation and miR-152 overexpression both reversed the effects of miR-152 inhibition on the NPC cells. In addition, miR-152 expression increased as a result of the inhibition of the methylation level of miR-152 when DNMT1 expression was downregulated.\nCONCLUSION: The overexpression of miR-152 inhibited the migration and invasion of NPC cells by targeting DNMT1. Furthermore, DNMT1 regulated miR-152 expression through DNA methylation. Overall, the novel miR-152-DNMT1 regulatory circuit may provide better understanding of the pathogenesis of NPC and new epigenetic therapeutic target in NPC.", "claims": [{"id": "claim-method", "statement": "Student’s t-test and one-way ANOVA were performed in GraphPad Prism 5.0 software and SPSS 13.0.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29628766", "locator": "sections[16] \"Statistical analysis\" paragraph 2", "quote": "Student’s t-test and one-way ANOVA were performed in GraphPad Prism 5.0 software and SPSS 13.0."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The MiR-152 expression profiles in NPC tissues were obtained from Gene Expression Omnibus (GEO GSE36682; www.ncbi.nlm.nih.gov/geo/).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29628766", "locator": "sections[3] \"Patients and samples\" paragraph 2", "quote": "The MiR-152 expression profiles in NPC tissues were obtained from Gene Expression Omnibus (GEO GSE36682; www.ncbi.nlm.nih.gov/geo/)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The paper used Student’s t-test and one-way ANOVA, with differences considered significant at P<0.05 and data reported as the mean and standard deviation from at least three separate experiments; NPC tissue miR-152 expression profiles were obtained from Gene Expression Omnibus dataset GSE36682 and compared using GEO2R. 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