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pmid:29682102
Expression of Pentose Phosphate Pathway-Related Proteins in Breast Cancer.
Junjeong Choi · Eun-Sol Kim · Ja Seung Koo
Disease markers · 2018 doi:10.1155/2018/9369358
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Experiment
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Complete file
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G6PDH positivity was associated with ER negativity (p = 0.001), PR negativity (p = 0.001), and HER-2 positivity (p < 0.001), whereas 6PGL positivity was associated with higher T stage (p = 0.004). The 562 expression profile from the TCGA database revealed increased expression of G6PDH and 6PG in the tumor compared with normal adjacent breast tissue. The expression of G6PDH was highest in HER-2 type. HER-2 and basal-like subtypes showed higher expression of 6PGDH than luminal types.\nCONCLUSION: PPP-related proteins are differentially expressed in breast cancer according to molecular subtype, and higher expression of G6PDH and 6PGL was noted in HER-2 subtype.", "claims": [{"id": "claim-method", "statement": "Multivariate regression analysis was performed using the Cox proportional hazard model.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29682102", "locator": "sections[9] \"2.7. 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Validation of Expression of PPP-Related Markers in a Public Database\" paragraph 2", "quote": "We obtained clinical information and level 3 normalized gene expression (RSEM) values from RNA sequencing data of breast cancer (BRCA) from the Broad Genome Data Analysis Center (GDAC) Firehose server (version 01-28-2016) and filtered out genes with an expression equal to zero in more than 50% of samples."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:29682102"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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