Proteomic analysis of pitcher fluid from Nepenthes × ventrata.

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We investigated protein profile in the pitcher fluid during the first three days of opening to understand carnivory trait of Nepenthes × ventrata. The proteome analysis of pitcher fluid from N. × ventrata was performed by label-free quantitative liquid chromatography mass spectrometry (nLC-MS/MSALL). Raw MS data have been deposited to the ProteomeXchange with identifier PXD007251. This dataset allows the identification and quantification of proteins from pitcher fluids to elucidate proteins involved in carnivory physiology of Nepenthes species.", "claims": [{"id": "claim-method", "statement": "Peptide detected was filtered by false discovery rate, FDR < 0.001.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29876422", "locator": "sections[11] \"Mass spectrometry and data analysis\" paragraph 2", "quote": "Peptide detected was filtered by false discovery rate, FDR < 0.001."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data are available via ProteomeXchange with identifier PXD007251 (http://www.ebi.ac.uk/pride/archive/projects/PXD007251)", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29876422", "locator": "sections[2] \"table-wrap\" paragraph 1", "quote": "Data are available via ProteomeXchange with identifier PXD007251 (http://www.ebi.ac.uk/pride/archive/projects/PXD007251)"}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study used label-free quantitative nLC-MS/MS proteomics with spectra filtered at FDR < 0.001, and deposited the resulting raw and processed source data in ProteomeXchange via PRIDE under identifier PXD007251.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:29876422", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The proteome analysis of pitcher fluid from N. × ventrata was performed by label-free quantitative liquid chromatography mass spectrometry (nLC-MS/MSALL)."}, {"asset": "source:pmid:29876422", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Raw MS data have been deposited to the ProteomeXchange with identifier PXD007251."}, {"asset": "source:pmid:29876422", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "This dataset allows the identification and quantification of proteins from pitcher fluids to elucidate proteins involved in carnivory physiology of Nepenthes species."}, {"asset": "source:pmid:29876422", "locator": "sections[11] \"Mass spectrometry and data analysis\" paragraph 2", "quote": "All spectra generated from IDA acquisitions were searched against the predicted peptide database from N. × ventrata reference transcriptome [2] using ProteinPilot™ v4.5 (AB SCIEX) for protein and peptide identifications."}, {"asset": "source:pmid:29876422", "locator": "sections[11] \"Mass spectrometry and data analysis\" paragraph 2", "quote": "Peptide detected was filtered by false discovery rate, FDR < 0.001."}, {"asset": "source:pmid:29876422", "locator": "sections[7] \"Data\" paragraph 2", "quote": "The mass spectrometry proteomics data of three technical replicates each were deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE [5] partner repository, with the dataset identifier PXD007251."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study used label-free quantitative nLC-MS/MS proteomics with spectra filtered at FDR < 0.001, and deposited the resulting raw and processed source data in ProteomeXchange via PRIDE under identifier PXD007251.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:29876422"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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