Time-course proteomics dataset to monitor protein-bound methionine oxidation in Bacillus cereus ATCC 14579.

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Methionine sulfoxide reductases catalyze the reduction of methionine sulfoxide to methionine in proteins. Here, we use high-throughput nanoLC-MS/MS methodology to establish detailed maps of oxidized proteins from Bacillus cereus ATCC 14579 ΔpBClin15 and its mutant for which the methionine sulfoxide reductase AB gene (msrAB) has been inactivated (Madeira et al., 2017) [1]. Lists of oxidized peptides and proteins identified at early exponential, late exponential and stationary growth phases are supplied in this article as data files. Raw data are deposited at the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers, PXD006169 and PDX006205 (http://www.ebi.ac/uk). Given the importance of methionine oxidation in several key cellular processes and its impact in the field of medical and food microbiology, this paper should be useful for further insightful redox studies in B. cereus and its numerous relatives.", "claims": [{"id": "claim-method", "statement": "Hierarchical clustering of oxidized proteins was carried out with PermutMatrix Software [11].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29896523", "locator": "sections[9] \"LC–MS/MS and data analysis\" paragraph 2", "quote": "Hierarchical clustering of oxidized proteins was carried out with PermutMatrix Software [11]."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Raw LC–MS/MS files are available via the PRIDE [3] partner repository with the dataset identifier PXD006169 (cellular proteome) and PXD00006205 (exoproteome).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:29896523", "locator": "sections[5] \"Data\" paragraph 2", "quote": "Raw LC–MS/MS files are available via the PRIDE [3] partner repository with the dataset identifier PXD006169 (cellular proteome) and PXD00006205 (exoproteome)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:29896523"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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