A novel transcriptional network for the androgen receptor in human epididymis epithelial cells.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:30016502", "kind": "science-record", "revision": 2, "content_hash": "sha256:5a5e93bb32a3811dd3ac998d47c8846dd74cfaafa1f84bfab333b042a932c4be", "created_at": "2026-11-01T15:40:35Z", "updated_at": "2026-11-01T18:49:41Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "A novel transcriptional network for the androgen receptor in human epididymis epithelial cells.", "doi": "10.1093/molehr/gay029", "pmid": "30016502", "authors": ["Rui Yang", "James A Browne", "Scott E Eggener", "Shih-Hsing Leir", "Ann Harris"], "journal": "Molecular human reproduction", "year": 2018, "abstract": "STUDY QUESTION: What is the transcriptional network governed by the androgen receptor (AR) in human epididymis epithelial (HEE) cells from the caput region and if the network is tissue-specific, how is this achieved?\nSUMMARY ANSWER: About 200 genes are differentially expressed in the caput HEE cells after AR activation; the AR transcriptional network is tissue-specific and may be mediated in part by distinct AR co-factors including CAAT-enhancer binding protein beta (CEBPB) and runt-related transcription factor 1 (RUNX1).\nWHAT IS KNOWN ALREADY: Little is known about the AR transcriptional program genome wide in HEE cells, nor its co-factors in those cells. AR has been best studied in the prostate gland epithelium and prostate cancer cell lines, due to the important role of this factor in prostate cancer. However AR-associated differentially expressed genes (DEGs) and AR co-factors have not yet been compared between human epididymis and prostate epithelial cells.\nSTUDY DESIGN, SIZE, DURATION: Caput HEE cells from two donors were exposed to the synthetic androgen R1881 at 1 nM for 12-16 h after 72 h of hormone starvation.\nPARTICIPANTS/MATERIALS, SETTING, METHODS: Chromatin was prepared from R1881-treated and vehicle control HEE cells. AR-associated chromatin was purified by chromatin immunoprecipitation (ChIP) and AR occupancy genome wide was revealed by deep sequencing (ChIP-seq). Two independent biological replicates were performed. Total RNA was prepared from R1881 and control-treated HEE cells and gene expression profiles were documented by RNA-seq. The interaction of the potential novel AR co-factors CEBPB and RUNX1, identified through in-silico motif analysis of AR ChIP-seq data, was examined by ChIP-qPCR after siRNA-mediated depletion of each co-factor individually or simultaneously.\nMAIN RESULTS AND THE ROLE OF CHANCE: The results identify about 200 genes that are differentially expressed (DEGs) in HEE cells after AR activation. Some of these DEGs show occupancy of AR at their promoters or cis-regulatory elements suggesting direct regulation. However, there is little overlap in AR-associated DEGs between HEE and prostate epithelial cells. Inspection of over-represented motifs in AR ChIP-seq peaks identified CEBPB and RUNX1 as potential co-factors, with no evidence for FOXA1, which is an important co-factor in the prostate epithelium. CEBPB and RUNX1 ChIP-seq in HEE cells showed that both these factors often occupied AR-binding sites, though rarely simultaneously. Further analysis at a single AR-regulated locus (FK506-binding protein 5, FKPB5) suggests that CEBPB may be a co-activator. These data suggest a novel AR transcriptional network governs differentiated functions of the human epididymis epithelium.\nLARGE SCALE DATA: AR ChIP-seq and RNA-seq data are deposited at GEO: GSE109063.\nLIMITATIONS, REASONS FOR CAUTION: There is substantial donor-to-donor variation in primary HEE cells cultures. We applied stringent statistical tests with a false discovery rate (FDR) of 0.1% for ChIP-seq and standard pipelines for RNA-seq so it is possible that we have missed some AR-regulated genes that are important in caput epididymis function.\nWIDER IMPLICATIONS OF THE FINDINGS: Our data suggest that a novel AR transcriptional network governs differentiated functions of the human epididymis epithelium. Since this cell layer has a critical role in normal sperm maturation, the results are of broader significance in understanding the mechanisms underlying the maintenance of fertility in men.\nSTUDY FUNDING/COMPETING INTERESTS: This work was funded by the National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Development: R01 HD068901 (PI: Harris). The authors have no competing interests to declare.", "claims": [{"id": "claim-target-result", "statement": "The study used stringent statistical tests with a 0.1% false discovery rate for ChIP-seq and standard RNA-seq pipelines, and the source AR ChIP-seq and RNA-seq data are available at GEO under accession GSE109063.", "kind": "quantitative", "target": {"value": 0.1, "unit": "per cent", "tolerance": null, "source": "There is substantial donor-to-donor variation in primary HEE cells cultures. We applied stringent statistical tests with a false discovery rate (FDR) of 0.1% for ChIP-seq and standard pipelines for RNA-seq so it is possible that we have missed some AR-regulated genes that are important in caput epididymis function."}, "evidence": [{"asset": "source:pmid:30016502", "locator": "sections[0] \"Abstract\" paragraph 6", "quote": "The results identify about 200 genes that are differentially expressed (DEGs) in HEE cells after AR activation."}, {"asset": "source:pmid:30016502", "locator": "sections[0] \"Abstract\" paragraph 8", "quote": "There is substantial donor-to-donor variation in primary HEE cells cultures. We applied stringent statistical tests with a false discovery rate (FDR) of 0.1% for ChIP-seq and standard pipelines for RNA-seq so it is possible that we have missed some AR-regulated genes that are important in caput epididymis function."}, {"asset": "source:pmid:30016502", "locator": "sections[0] \"Abstract\" paragraph 7", "quote": "AR ChIP-seq and RNA-seq data are deposited at GEO: GSE109063."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The study used stringent statistical tests with a 0.1% false discovery rate for ChIP-seq and standard RNA-seq pipelines, and the source AR ChIP-seq and RNA-seq data are available at GEO under accession GSE109063.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:30016502"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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