Integrated Dissection of Cysteine Oxidative Post-translational Modification Proteome During Cardiac Hypertrophy.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:30141336", "kind": "science-record", "revision": 4, "content_hash": "sha256:255dcf45d42481d5843251be960a8acbcd6c8d15cb8fb66625f0ce39d6e2dd68", "created_at": "2026-11-01T17:32:21Z", "updated_at": "2026-11-01T22:43:30Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Integrated Dissection of Cysteine Oxidative Post-translational Modification Proteome During Cardiac Hypertrophy.", "doi": "10.1021/acs.jproteome.8b00372", "pmid": "30141336", "authors": ["Jie Wang", "Howard Choi", "Neo C Chung", "Quan Cao", "Dominic C M Ng", "Bilal Mirza", "Sarah B Scruggs", "Ding Wang", "Anders O Garlid", "Peipei Ping"], "journal": "Journal of proteome research", "year": 2018, "abstract": "Cysteine oxidative modification of cellular proteins is crucial for many aspects of cardiac hypertrophy development. However, integrated dissection of multiple types of cysteine oxidative post-translational modifications (O-PTM) of proteomes in cardiac hypertrophy is currently missing. Here we developed a novel discovery platform that encompasses a customized biotin switch-based quantitative proteomics pipeline and an advanced analytic workflow to comprehensively profile the landscape of cysteine O-PTM in an ISO-induced cardiac hypertrophy mouse model. Specifically, we identified a total of 1655 proteins containing 3324 oxidized cysteine sites by at least one of the following three modifications: reversible cysteine O-PTM, cysteine sulfinylation (CysSO2H), and cysteine sulfonylation (CysSO3H). Analyzing the hypertrophy signatures that are reproducibly discovered from this computational workflow unveiled four biological processes with increased cysteine O-PTM. Among them, protein phosphorylation, creatine metabolism, and response to elevated Ca2+ pathways exhibited an elevation of cysteine O-PTM in early stages, whereas glucose metabolism enzymes were increasingly modified in later stages, illustrating a temporal regulatory map in cardiac hypertrophy. Our cysteine O-PTM platform depicts a dynamic and integrated landscape of the cysteine oxidative proteome, through the extracted molecular signatures, and provides critical mechanistic insights in cardiac hypertrophy. Data are available via ProteomeXchange with identifier PXD010336.", "claims": [{"id": "claim-method", "statement": "The significance of abundance change in ISO and Vehicle conditions was evaluated by a paired two-sided t-test. Pearson correlation was performed to analyze the association between phenotype and abundance of Mal-biotin labeled cysteines.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:30141336", "locator": "sections[14] \"Additional statistical analysis\" paragraph 2", "quote": "The significance of abundance change in ISO and Vehicle conditions was evaluated by a paired two-sided t-test. Pearson correlation was performed to analyze the association between phenotype and abundance of Mal-biotin labeled cysteines."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data are available via ProteomeXchange with identifier PXD010336.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:30141336", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Data are available via ProteomeXchange with identifier PXD010336."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Specifically, we identified a total of 1,655 proteins containing 3,324 oxidized cysteine sites by at least one of the following three modifications: reversible cysteine O-PTM, cysteine sulfinylation (CysSO2H), and cysteine sulfonylation (CysSO3H).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:30141336", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Specifically, we identified a total of 1,655 proteins containing 3,324 oxidized cysteine sites by at least one of the following three modifications: reversible cysteine O-PTM, cysteine sulfinylation (CysSO2H), and cysteine sulfonylation (CysSO3H)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Specifically, we identified a total of 1,655 proteins containing 3,324 oxidized cysteine sites by at least one of the following three modifications: reversible cysteine O-PTM, cysteine sulfinylation (CysSO2H), and cysteine sulfonylation (CysSO3H).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:30141336"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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