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pmid:30208918

Time-course mass spectrometry data of adipose mesenchymal stem cells acquiring chondrogenic phenotype.

Jeffrey R Janus · Stephen G Voss · Benjamin J Madden · Mary Cristine Charlesworth · Michael S Oldenburg · Dale Ekbom · Serban San-Marina

BMC research notes · 2018 doi:10.1186/s13104-018-3750-6

Paper

complete

Experiment

partial

Data

partial

Analysis

partial

Execution

absent

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Record details sci_version · id · kind · revision · content_hash · schema · created_at · updated_at · generator
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pmid:30208918
kind
science-record
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2026-11-01T15:40:37Z
updated_at
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generator object · 2 fields
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paper object · 10 fields
state
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title
Time-course mass spectrometry data of adipose mesenchymal stem cells acquiring chondrogenic phenotype.
doi
10.1186/s13104-018-3750-6
pmid
30208918
authors array · 7 items
[0]
Jeffrey R Janus
[1]
Stephen G Voss
[2]
Benjamin J Madden
[3]
Mary Cristine Charlesworth
[4]
Michael S Oldenburg
[5]
Dale Ekbom
[6]
Serban San-Marina
journal
BMC research notes
year
2018
abstract
OBJECTIVES: Rabbit adipose mesenchymal stem cells were used for the purpose of studying acquisition of the chondrogenic phenotype over time at 1, 14 and 28 days after in vitro incubation with differentiation media, using nano-liquid chromatography electrospray ionization tandem mass spectrometry analysis. This was part of a preliminary study of the behavior of differentiated adipose stem cells for use in a rabbit model of laryngoplasty. DATA DESCRIPTION: The data comprise .MGF, .RAW, .MZID, and .XLSX, lists of peaks, peptides and proteins identified by nano-flow liquid chromatography electrospray ionization tandem mass spectrometry analysis upon incubation with non-differentiating (ND) or chondrogenic differentiating (CHD) media (ProteomeXchange ID PXD010236). XLSX files contain the following information: day 1 CT (control, N = 3499 proteins), day 14 ND (N = 3106 proteins), day 28 ND (N = 3116 proteins), day 14 CHD (N = 2901 proteins), and day 28 CHD (N = 2876 proteins). Proteins are characterized with respect to their - 10lgP value, percent coverage, number of total as well as unique peptides after trypsin digestion, derivatization method (carbamidomethylation, oxidation, or combined carbamidomethylation + oxidation), average mass, and include a full description.
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sections[5] "nLC–MS/MS analysis" paragraph 2
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Peptide IDs were accepted if they could be established at greater than 95% probability by the Scaffold Local FDR algorithm.
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MS/MS spectra file (.mgf)
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ND day 28
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MS/MS spectra file (.mgf)
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ftp://massive.ucsd.edu/MSV000082505/peak/day28ctrl.mgf
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[0]
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ND day 28
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Mass spectrometry data (.raw)
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ftp://massive.ucsd.edu/MSV000082505/raw/day28ctrl.raw
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ND day 28
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Peptide data (.mzid)
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ftp://massive.ucsd.edu/MSV000082505/result/day28ctrl_peptides_1_1_0.mzid
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CHD day 14
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MS/MS spectra file (.mgf)
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ftp://massive.ucsd.edu/MSV000082505/peak/day14ctrl.mgf
[11] array · 4 items
[0]
File 11
[1]
CHD day 14
[2]
Mass spectrometry data (.raw)
[3]
ftp://massive.ucsd.edu/MSV000082505/raw/day14chond.raw
[12] array · 4 items
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File 12
[1]
CHD day 14
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Peptide data (.mzid)
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ftp://massive.ucsd.edu/MSV000082505/result/day14chond_peptides_1_1_0.mzid
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[0]
File 13
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CHD day 28
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MS/MS spectra file (.mgf)
[3]
ftp://massive.ucsd.edu/MSV000082505/peak/day28chond.mgf
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[0]
File 14
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CHD day 28
[2]
Mass spectrometry data (.raw)
[3]
ftp://massive.ucsd.edu/MSV000082505/raw/day28chond.raw
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[0]
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CHD day 28
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Peptide data (.mzid)
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ftp://massive.ucsd.edu/MSV000082505/result/day28chond_peptides_1_1_0.mzid
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[1]
Day 1 Ctrl
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Protein list file (.xlsx)
[3]
ftp://massive.ucsd.edu/MSV000082505/updates/2018-07-19_ssanmarina1_eec23ce3/other/Day1_ctrl_proteins.xlsx
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File 17
[1]
ND day 14
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Protein list file (.xlsx)
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ftp://massive.ucsd.edu/MSV000082505/updates/2018-07-19_ssanmarina1_eec23ce3/other/Day14_ctrl_proteins.xlsx
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CHD day 14
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Protein list file (.xlsx)
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ftp://massive.ucsd.edu/MSV000082505/updates/2018-07-19_ssanmarina1_eec23ce3/other/Day14_chondro_proteins.xlsx
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ND day 28
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Protein list file (.xlsx)
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ftp://massive.ucsd.edu/MSV000082505/updates/2018-07-19_ssanmarina1_eec23ce3/other/Day28_ctrl_proteins.xlsx
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CHD day 28
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Protein list file (.xlsx)
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section[5]/table-wrap[1]#Tab1
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Peptide IDs were accepted if they could be established at greater than 95% probability by the Scaffold Local FDR algorithm.
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Time-course mass spectrometry data of adipose mesenchymal stem cells acqui
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This was part of a preliminary study of the behavior of differentiated adipose stem cells for use in a rabbit model of laryngoplasty.\nDATA DESCRIPTION: The data comprise .MGF, .RAW, .MZID, and .XLSX, lists of peaks, peptides and proteins identified by nano-flow liquid chromatography electrospray ionization tandem mass spectrometry analysis upon incubation with non-differentiating (ND) or chondrogenic differentiating (CHD) media (ProteomeXchange ID PXD010236). XLSX files contain the following information: day 1 CT (control, N = 3499 proteins), day 14 ND (N = 3106 proteins), day 28 ND (N = 3116 proteins), day 14 CHD (N = 2901 proteins), and day 28 CHD (N = 2876 proteins). 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