Nucleolar and Spindle Associated Protein 1 (NUSAP1) Inhibits Cell Proliferation and Enhances Susceptibility to Epirubicin In Invasive Breast Cancer Cells by Regulating Cyclin D Kinase (CDK1) and DLGAP5 Expression.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:30476929", "kind": "science-record", "revision": 7, "content_hash": "sha256:8bdce9637e9c81772f59bd72903b1065497569ea2a790983e64ff69d0992f710", "created_at": "2026-11-01T15:40:39Z", "updated_at": "2026-11-01T21:21:47Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Nucleolar and Spindle Associated Protein 1 (NUSAP1) Inhibits Cell Proliferation and Enhances Susceptibility to Epirubicin In Invasive Breast Cancer Cells by Regulating Cyclin D Kinase (CDK1) and DLGAP5 Expression.", "doi": "10.12659/MSM.910364", "pmid": "30476929", "authors": ["Xi Zhang", "Yuliang Pan", "Huiqun Fu", "Juan Zhang"], "journal": "Medical science monitor : international medical journal of experimental and clinical research", "year": 2018, "abstract": "BACKGROUND Differentially expressed genes (DEGs) of IBC were selected from the Gene Expression Omnibus (GEO) chip data: GSE21422 and GSE21974. Network analysis of the DEGs and IBC-related genes was performed in STRING database to find the core gene. Thus, this study aimed to determine the role of NUSAP1 in invasive breast cancer (IBC) and to investigate its effect on drug susceptibility to epirubicin (E-ADM). MATERIAL AND METHODS The mRNA expression of NUSAP1 was determined by quantitative polymerase chain reaction (q-PCR). The protein expression was detected by Western blotting. Cell growth and growth cycle were detected by MTT assay and flow cytometry, respectively. Cell migration and invasion were tested by Transwell assay. RESULTS Through use of gene network analysis, we found that NUSAP1 interacts with IBC-related genes. NUSAP1 presented high expression in IBC tissue samples and MCF-7 cells. NUSAP1 overexpression promoted the growth, migration, and invasion of MCF-7 cells. While NUSAP1 gene silencing downregulated the expression of genes associated with cell cycle progression in G2/M phase, cyclin D kinase (CDK1) and DLGAP5 arrested cells in G2/M phase and significantly inhibited the growth, migration, and invasion of MCF-7 cells. si-NUSAP1 increased the susceptibility of MCF-7 cells to E-ADM-induced apoptosis. CONCLUSIONS Our study provides evidence that downregulation of NUSAP1 can inhibit the proliferation, migration, and invasion of IBC cells by regulating CDK1 and DLGAP5 expression and enhances the drug susceptibility to E-ADM.", "claims": [{"id": "claim-method", "statement": "Measurement data are expressed as mean ± standard deviation (SD) and were analyzed by t test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:30476929", "locator": "sections[15] \"Statistical analysis\" paragraph 2", "quote": "Measurement data are expressed as mean ± standard deviation (SD) and were analyzed by t test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data set GSE21974 was obtained from the Gene Expression Omnibus (GEO) (http://www.nebi.nlm.nih.gov/geo) with “invasive breast cancer” and/or “neoadjuvant chemotherapy” as the key words. The chip platform was Agilent-014850 Whole Human Genome Microarray 4×44k G4112F (GPL 6480). GSE21974 included 57 samples (32 invasive breast cancer samples before treatment and 25 invasive breast cancer samples after treatment).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:30476929", "locator": "sections[3] \"Gene chip data processing\" paragraph 2", "quote": "The data set GSE21974 was obtained from the Gene Expression Omnibus (GEO) (http://www.nebi.nlm.nih.gov/geo) with “invasive breast cancer” and/or “neoadjuvant chemotherapy” as the key words. The chip platform was Agilent-014850 Whole Human Genome Microarray 4×44k G4112F (GPL 6480). 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The protein expression was detected by Western blotting. Cell growth and growth cycle were detected"], "order_basis": "paper_text_order:738", "id": "assay:1"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["sceptibility of MCF-7 cells to E-ADM-induced apoptosis. CONCLUSIONS Our study provides evidence tha", "ell growth and growth cycle were detected by MTT assay and flow cytometry, respectively. Cell"], "order_basis": "paper_text_order:802", "id": "assay:2"}, {"role": "data_generation", "definition_id": "flow_cytometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["growth cycle were detected by MTT assay and flow cytometry, respectively. Cell migration and invasion w"], "order_basis": "paper_text_order:817", "id": "assay:3"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ed high expression in IBC tissue samples and MCF-7 cells. NUSAP1 overexpression promoted the gr", "moted the growth, migration, and invasion of MCF-7 cells. While NUSAP1 gene silencing downregul", "bited the growth, migration, and invasion of MCF-7 cells. si-NUSAP1 increased the susceptibilit", "s. si-NUSAP1 increased the susceptibility of MCF-7 cells to E-ADM-induced apoptosis. CONCLUSION"], "order_basis": "paper_text_order:1066", "id": "assay:4"}, {"role": "data_generation", "definition_id": "microarray", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["atform was Agilent-014850 Whole Human Genome Microarray 4×44k G4112F (GPL 6480). GSE21974 included 5"], "order_basis": "paper_text_order:2026", "id": "assay:5"}]}