Genomics of neonatal sepsis: has-miR-150 targeting BCL11B functions in disease progression.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:30497506", "kind": "science-record", "revision": 8, "content_hash": "sha256:2067b13bcb56035ef2d6b884b3bacecbcf33a20ae45c3e1d0e0996e685d129ae", "created_at": "2026-11-01T15:40:39Z", "updated_at": "2026-11-01T22:43:39Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Genomics of neonatal sepsis: has-miR-150 targeting BCL11B functions in disease progression.", "doi": "10.1186/s13052-018-0575-9", "pmid": "30497506", "authors": ["Li Huang", "Lixing Qiao", "Huan Zhu", "Li Jiang", "Liping Yin"], "journal": "Italian journal of pediatrics", "year": 2018, "abstract": "BACKGROUND: Neonatal sepsis is an inflammatory systemic syndrome, which is a major cause of morbidity and mortality in premature infants. We analyzed the expression profile data of E-MTAB-4785 to reveal the pathogenesis of the disease.\nMETHODS: The expression profile dataset E-MTAB-4785, which contained 17 sepsis samples and 19 normal samples, was obtained from the ArrayExpress database. The differentially expressed genes (DEGs) were analyzed by the Bayesian testing method in limma package. Based on the DAVID online tool, enrichment analysis was conducted for the DEGs. Using STRING database and Cytoscape software, protein-protein interaction (PPI) network and module analyses were performed. Besides, transcription factor (TF)-DEG regulatory network was also constructed by Cytoscape software. Additionally, miRNA-DEG pairs were searched using miR2Disease and miRWalk 2.0 databases, followed by miRNA-DEG regulatory network was visualized by Cytoscape software.\nRESULTS: A total of 275 DEGs were identified from the sepsis samples in comparison to normal samples. TSPO, MAPK14, and ZAP70 were the hub nodes in the PPI network. Pathway enrichment analysis indicated that CEBPB and MAPK14 were enriched in TNF signaling pathway. Moreover, CEBPB and has-miR-150 might function in neonatal sepsis separately through targeting MAPK14 and BCL11B in the regulatory networks. These genes and miRNA might be novel targets for the clinical treatment of neonatal sepsis.\nCONCLUSION: TSPO, ZAP70, CEBPB targeting MAPK14, has-miR-150 targeting BCL11B might affect the pathogenesis of neonatal sepsis. However, their roles in neonatal sepsis still needed to be confirmed by further experimental researches.", "claims": [{"id": "claim-method", "statement": "The Bayesian testing method [16] in limma package (http://www.bioconductor.org/packages/release/bioc/html/limma.html) [17] was utilized to identify the DEGs between the two group samples. The p-values were conducted with multiple testing adjustment based on the Benjamini & Hochberg method [18].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:30497506", "locator": "sections[4] \"Data preprocessing and differentially expressed genes (DEGs) screening\" paragraph 2", "quote": "The Bayesian testing method [16] in limma package (http://www.bioconductor.org/packages/release/bioc/html/limma.html) [17] was utilized to identify the DEGs between the two group samples. The p-values were conducted with multiple testing adjustment based on the Benjamini & Hochberg method [18]."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:30497506", "locator": "sections[16] \"ack\" paragraph 1", "quote": "The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:30497506"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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