Gene ontology analysis of transcriptome data from DMBA-induced mammary tumors of rats fed a high-corn oil and a high-extra virgin olive oil diet.

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In this work we present data in relation to the transcriptional effects of two high-fat diets, one high in corn oil (HCO) and one high in extra-virgin olive oil (HOO), administered from weaning or after induction, on 7,12-dimethylbenz(a)anthracene (DMBA)-induced rat mammary tumors. Raw data were deposited at ArrayExpress under accession number E-MTAB-3541. We compared the gene expression profiles of the mammary tumors from the high-fat diet groups with those from the control group, finding different effects of diets depending on timing and type of dietary intervention. Lists of differentially expressed genes were analyzed to find overrepresented categories of biological significance. Here we provide information about the cell functions categories overrepresented in significantly modulated genes by effect of the high-fat diets. Further investigations of such functions are described in \"A high corn oil diet strongly stimulates mammary carcinogenesis, while a high extra virgin olive oil diet has a weak effect, through changes in metabolism, immune system function, and proliferation/apoptosis pathways\" (Escrich et al., in press) [1].", "claims": [{"id": "claim-method", "statement": "Statistical analysis of data was carried out using language R (www.bioconductor.org), and selection of genes differentially expressed between conditions was based on a linear model with empirical Bayes moderation [6]. Genes were classified into ranks according to its differential expression. 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Raw data were deposited at A", "rimental design Female Sprague-Dawley Crl:SD rats were obtained at 23 days of age from Charles Rive", "in 1 mL of corn oil. At the end of the assay rats were euthanized by decapitation on days 236–256."], "order_basis": "paper_text_order:36", "id": "assay:1"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.69, "evidence": ["m, immune system function, and proliferation/apoptosis pathways\" (Escrich et al., in press) [1]."], "order_basis": "paper_text_order:1342", "id": "assay:2"}]}