Recognition of HER2 expression in hepatocellular carcinoma and its significance in postoperative tumor recurrence.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:30714677", "kind": "science-record", "revision": 7, "content_hash": "sha256:7f4cba36147b527dcff105cf83071b34b33d005fda88441afc7eecd7852bef54", "created_at": "2026-11-01T15:40:41Z", "updated_at": "2026-11-01T21:21:57Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Recognition of HER2 expression in hepatocellular carcinoma and its significance in postoperative tumor recurrence.", "doi": "10.1002/cam4.2006", "pmid": "30714677", "authors": ["Ji-Hua Shi", "Wen-Zhi Guo", "Yang Jin", "Hua-Peng Zhang", "Chun Pang", "Jie Li", "Pål-Dag Line", "Shui-Jun Zhang"], "journal": "Cancer medicine", "year": 2019, "abstract": "BACKGROUND: The ERBB2 oncogene hypothesis is challenged in hepatocellular carcinoma (HCC) with the conflicting evidences of human epidermal growth factor receptor 2 (HER2) overexpression. HER2 could be a new target as a treatment option for HCC as well as tumor recurrence after surgery. HER2 in HCC biology needs further explorations.\nMETHODS: Clinical and mRNA data of HCC patients were obtained from TCGA HCC cohort, GSE89377 and GSE115018. Western Blotting and immunohistochemistry were employed to test expression of HER2, E-cadherin, and Vimentin. In HepG2, JM1, HER2-transfected McA cells, and TGF-β cocultured JM1 cells, HCC biology, including cell survival, proliferation, and epithelial-to-mesenchymal transition (EMT) phenotypes were evaluated.\nRESULTS: ERBB2 mRNA amplification was found in HCC datasets, and its expression was downregulated in high grade HCC with a worse overall survival. HER2 overexpression was identified in H4IIE, HepG2, JM1 cells, and 82% (14/17) HCC samples, and tumor stage was correlated with expression of HER2, E-cadherin, and Vimentin (P < 0.05). Trastuzumab with the high concentrations suppressed proliferation of HER2-positive hepatoma cells (P < 0.05); in the coculture model to induce EMT of JM1 cells, HER2 expression increased with downregulated E-cadherin and upregulated Vimentin. Trastuzumab intravenous injection inhibited in vivo tumor size and metastases (P < 0.05). Signal analysis revealed that HER2 functioned through upregulation of β-catenin and inhibition of SMAD3.\nCONCLUSION: HER2 expression pattern is linked with tumor stage and overall survival; the transforming function of HER2 is found more relevant through β-catenin and SMAD3. 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Western Blotting and immunohistochemistry were employed to te", "patients with immunohistochemistry (IHC) and western blotting (WB). Breast cancer sample, which was confir"], "order_basis": "paper_text_order:514", "id": "assay:1"}, {"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["GSE89377 and GSE115018. Western Blotting and immunohistochemistry were employed to test expression of HER2, E-", "he archive materials of 17 HCC patients with immunohistochemistry (IHC) and western blotting (WB). Breast canc", "f 17 HCC patients with immunohistochemistry (IHC) and western blotting (WB). Breast cancer sa"], "order_basis": "paper_text_order:535", "id": "assay:2"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ession of HER2, E-cadherin, and Vimentin. In HepG2, JM1, HER2-transfected McA cells, and TGF-β", "HER2 overexpression was identified in H4IIE, HepG2, JM1 cells, and 82% (14/17) HCC samples, and", "HCC, we selected hepatoma cell lines (H4IIE, HepG2, JM1, McA‐RH7777) and the archive materials"], "order_basis": "paper_text_order:627", "id": "assay:3"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.85, "evidence": ["-cadherin, and Vimentin. In HepG2, JM1, HER2-transfected McA cells, and TGF-β cocultured JM1 cells, H"], "order_basis": "paper_text_order:644", "id": "assay:4"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Trastuzumab intravenous injection inhibited in vivo tumor size and metastases (P < 0.05). Signal", "d 9‐12 weeks, were used for the experiments. In vivo inoculation of JM1/C hepatoma cells in the s", "sitive,20 were used as positive control. 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