A Long Noncoding RNA (lncRNA)-Associated Competing Endogenous RNA (ceRNA) Network Identifies Eight lncRNA Biomarkers in Patients with Osteoarthritis of the Knee.

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:30890688", "kind": "science-record", "revision": 4, "content_hash": "sha256:d5ad365ff933191d759114bdb862374a76523526705b32e9a97c54a87d3fd86e", "created_at": "2026-11-01T15:40:43Z", "updated_at": "2026-11-01T21:22:04Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "A Long Noncoding RNA (lncRNA)-Associated Competing Endogenous RNA (ceRNA) Network Identifies Eight lncRNA Biomarkers in Patients with Osteoarthritis of the Knee.", "doi": "10.12659/MSM.915555", "pmid": "30890688", "authors": ["Yuxi Chen", "Yu Lin", "Ye Bai", "Daolin Cheng", "Zhenggang Bi"], "journal": "Medical science monitor : international medical journal of experimental and clinical research", "year": 2019, "abstract": "BACKGROUND Osteoarthritis (OA) of the knee is a common disease that is associated with chronic pain. This study aimed to identify and investigate the functional role of biomarkers associated with long noncoding RNA (lncRNA) in the progression of OA of the knee by lncRNA-associated competing endogenous RNA (ceRNA) integrated network analysis. MATERIAL AND METHODS High-quality microRNA (miRNA)-lncRNA and miRNA-mRNA interactions and lncRNA and mRNA expression profiles for patients with OA of the knee with mild and severe pain were obtained from the Gene Expression Omnibus (GEO) database (GSE99662). A three-step computational method was used to construct the lncRNA-associated ceRNA interaction network in OA by integrating miRNA-lncRNA/mRNA interactions and lncRNA/mRNA expression profiles in patients with OA with mild and severe pain. RESULTS A total of 1,870 dysregulated lncRNA-mRNA interactions were obtained in the lncRNA-associated ceRNA network in OA, including 476 gain and 1,394 loss interactions, covering 131 lncRNAs and 1,251 mRNAs. Characterization of the lncRNA-associated ceRNA network in OA indicated that lncRNAs had roles in the network. Further differential expression analysis identified eight lncRNA biomarkers, which could distinguish between patients with OA with mild pain and severe pain. These lncRNA-associated interactions showed significantly different co-expression patterns in samples from patients with OA of the knee associated with mild pain. CONCLUSIONS Integrated network analysis of lncRNA-associated ceRNA identified eight lncRNA molecular biomarkers associated with the progression of OA of the knee.", "claims": [{"id": "claim-method", "statement": "Student’s t-test was used to evaluate the significance of differential expression of RNAs between samples of OA associated with mild pain and severe pain.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:30890688", "locator": "sections[6] \"Identification of differential expression of mRNA and lncRNA\" paragraph 2", "quote": "Student’s t-test was used to evaluate the significance of differential expression of RNAs between samples of OA associated with mild pain and severe pain."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Also, lncRNA and mRNA expression profiles for patients with osteoarthritis (OA) of the knee and with mild and severe pain were obtained from the Gene Expression Omnibus (GEO) database (accession GSE99662) [1].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:30890688", "locator": "sections[3] \"Data collection\" paragraph 2", "quote": "Also, lncRNA and mRNA expression profiles for patients with osteoarthritis (OA) of the knee and with mild and severe pain were obtained from the Gene Expression Omnibus (GEO) database (accession GSE99662) [1]."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:30890688"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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