Long Non-Coding RNA ZFAS1 as a Novel Potential Biomarker for Predicting the Prognosis of Thyroid Cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31012438", "kind": "science-record", "revision": 8, "content_hash": "sha256:c4283e93cd0851a88d40b1fc76360fea73bf2df53722e36cfd4b42da85568246", "created_at": "2026-11-01T15:40:44Z", "updated_at": "2026-11-01T22:00:30Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Long Non-Coding RNA ZFAS1 as a Novel Potential Biomarker for Predicting the Prognosis of Thyroid Cancer.", "doi": "10.12659/MSM.912921", "pmid": "31012438", "authors": ["Chuan-Gang Han", "Yan Huang", "Lu Qin"], "journal": "Medical science monitor : international medical journal of experimental and clinical research", "year": 2019, "abstract": "BACKGROUND Thyroid cancer is a type of endocrine cancers with rapidly increased incidence. Recent studies have indicated long non-coding RNAs (lncRNAs) played crucial roles in thyroid cancer tumorigenesis and progression. However, the roles of most lncRNAs in thyroid cancer were still unclear. MATERIAL AND METHODS We used TCGA (The Cancer Genome Atlas), GSE50901, GSE29265, and GSE33630 datasets to analyze the expression pattern of ZFAS1 (ZNFX1 antisense RNA 1). The correlation between ZFAS1 and clinicopathological features in thyroid cancer was analyzed. Cell proliferation and cell cycle assays were used to validate the roles of ZFAS1 in thyroid cancer cell lines. DAVID (the database for annotation, visualization and integrated discover) system was used to perform GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis. The starBase datasets and Cytoscape was used to perform ceRNA (competitive endogenous RNA) network. RESULTS We demonstrated ZFAS1 was highly expressed in thyroid cancer compared to normal samples. Moreover, upregulation of ZFAS1 was positively correlated with clinicopathological features and poor prognosis in thyroid cancer. Functional validation showed knockdown of ZFAS1 suppressed cell proliferation and cell cycle in thyroid cancer cells. Bioinformatics analysis showed ZFAS1 was associated with translation, rRNA processing, intra-Golgi vesicle-mediated transport, ribosome, and ubiquitin-mediated proteolysis. CONCLUSIONS Our study suggested ZFAS1 could serve as a biomarker for thyroid cancer.", "claims": [{"id": "claim-method", "statement": "The absolute value of the Pearson correlation coefficient ≥0.30 was selected as a cutoff to identified reliable ZFAS1-mRNA pairs.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31012438", "locator": "sections[16] \"Bioinformatics analysis of ZFAS1 in thyroid cancer\" paragraph 2", "quote": "The absolute value of the Pearson correlation coefficient ≥0.30 was selected as a cutoff to identified reliable ZFAS1-mRNA pairs."}], "state": "complete"}, {"id": "claim-data-access", "statement": "To determine the biological effect of ZFAS1, we analyzed TCGA dataset downloaded from cBioPortal [13,14] (http://www.cbioportal.org/), which included matched normal tissues (n=58) and thyroid cancer samples (n=510).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31012438", "locator": "sections[13] \"Long non-coding RNA ZFAS1 was upregulated in human thyroid cancer\" paragraph 2", "quote": "To determine the biological effect of ZFAS1, we analyzed TCGA dataset downloaded from cBioPortal [13,14] (http://www.cbioportal.org/), which included matched normal tissues (n=58) and thyroid cancer samples (n=510)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31012438"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE29265"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE29265"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:31012438", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.12659/MSM.912921", "sha256": 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Functional validation showed knockdown of ZFAS1 suppressed cell proliferation and c", "Material and Methods Cell culture and cell transfection CAL62 and SW579 were cultured in RPMI-1640 m", "ied incubator with 5% CO2. On the day before transfection, the cells were harvested and seeded at 5×10", "cells per well in a 6-well plate. 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