SNAI2 3'untranslated region promotes the invasion of ovarian cancer cells by inducing MARCKS expression.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31258754", "kind": "science-record", "revision": 8, "content_hash": "sha256:551879dbea8abee31f8bacaba9bca1764fce8ba0284b3258b44b491103bc4ab7", "created_at": "2026-11-01T15:40:46Z", "updated_at": "2026-11-01T22:00:39Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "SNAI2 3'untranslated region promotes the invasion of ovarian cancer cells by inducing MARCKS expression.", "doi": "10.7150/jca.29489", "pmid": "31258754", "authors": ["Jun Li", "Jieyu Wang", "Huiran Yue", "Xin Lu"], "journal": "Journal of Cancer", "year": 2019, "abstract": "Objective: Recently, accumulating evidence has indicated that the 3' untranslated regions (3'UTRs) of protein coding genes play critical roles in the progression of various cancers, including ovarian cancer. This study is aimed to identify the potential role of SNAI2-3'UTR in ovarain cancer progression. Study Design: First, we tried to explore the clinical significance of SNAI2 in ovarian cancer using TCGA and GSE26712 dataset. Then, gain-of-function studies were performed to establish the role of SNAI2-3'UTR in invasion and migration of ovarian cancer cells. Finally, efforts were made to identify the downstream targets of SNAI2-3'UTR. Results: Our data indicated that the expression of SNAI2 was significantly correlated with FIGO stage (P=0.015) and lymphatic invasion status (P=0.004), whereas not with age(P>0.05) and histological grade(P>0.05). Patients with higher SNAI2 expression had a shorter overall survival (OS) in both TCGA dataset (P=0.039, HR=1.54(1.02-2.33)) and GSE26712 dataset (P=0.0017, HR=1.77(1.24-2.54)). Functional studies revealed that SNAI2-3'UTR promoted the invasion of both OVCA433 and SKOV-3 cells without significantly affecting their migratory abilities. MARCKS, which was also involved in the invasion of ovarian cancer cells, was identified as a potential downstream target of SNAI2-3'UTR. SNAI2-3'UTR may function as a ceRNA to upregulate MARCKS expression in ovarian cancer. Conclusion: In conclusion, our study demonstrated that SNAI2-3'UTR cloud promote the invasion of ovarian cancer cells by upregulating MARCKS expression, which proposed a new mechanism by which SNAI2 contributed to progression of ovarian cancer.", "claims": [{"id": "claim-method", "statement": "The Log-rank test was used to determine the relationship between SNAI2 expression and clinical outcomes.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31258754", "locator": "sections[12] \"2.10 Statistical analysis\" paragraph 2", "quote": "The Log-rank test was used to determine the relationship between SNAI2 expression and clinical outcomes."}], "state": "complete"}, {"id": "claim-data-access", "statement": "We downloaded the level 3 Affymetrix HG-U133A gene expression data for SNAI2 and MARCKS from 208 patients with serous ovarian cancer in TCGA dataset to analyze the association of SNAI2 expression with the clinicopathological features, and to determine the correlation between SNAI2 expression and MARCKS expression.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31258754", "locator": "sections[3] \"2.1 TCGA datasets\" paragraph 2", "quote": "We downloaded the level 3 Affymetrix HG-U133A gene expression data for SNAI2 and MARCKS from 208 patients with serous ovarian cancer in TCGA dataset to analyze the association of SNAI2 expression with the clinicopathological features, and to determine the correlation between SNAI2 expression and MARCKS expression."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31258754"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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"note": "The relationship between SNAI2 expression and clinicopathological parameters in 208 primary ovarian cancer from TCGA datasets", "structure": {"pmcid": "PMC6584344", "section_index": 14, "section_title": "3.1 The relationship between SNAI2 expression and the clinicopathological parameters in ovarian cancer patients", "row_count": 14, "column_count": 5}, "table": {"label": "Table 1", "caption": "The relationship between SNAI2 expression and clinicopathological parameters in 208 primary ovarian cancer from TCGA datasets", "rows": [["Variables", "No. of patients", "SNAI2 expression", "P value"], ["Low, n(%)", "High, n(%)"], ["Age (years)", "", "", "", "0.325"], ["≤60", "121", "57(47.1%)", "64(52.9%)"], [">60", "87", "47(54.0%)", "40(46.0%)"], ["Histologic grade", "", "", "", "0.149"], ["G1/G2", "27", "17(63.0%)", "10(37.0%)"], ["G3", "181", "87(48.1%)", "94(51.9%)"], ["FIGO stage", "", "", "", "0.015"], ["I-II", "23", "17(73.9%)", "6(26.1%)"], ["III-IV", "185", "87(47.0%)", 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