Diagnostic and prognostic value of WNT family gene expression in hepatitis B virus‑related hepatocellular carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31322232", "kind": "science-record", "revision": 8, "content_hash": "sha256:3b08751857bf00cf2ae5dba2a94402cac0c92715af8f57b5142665046c6aca89", "created_at": "2026-11-01T15:40:47Z", "updated_at": "2026-11-01T22:44:06Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Diagnostic and prognostic value of WNT family gene expression in hepatitis B virus‑related hepatocellular carcinoma.", "doi": "10.3892/or.2019.7224", "pmid": "31322232", "authors": ["Quanfa Han", "Xiangkun Wang", "Xiwen Liao", "Chuangye Han", "Tingdong Yu", "Chengkun Yang", "Guanghui Li", "Bowen Han", "Ketuan Huang", "Guangzhi Zhu", "Zhengqian Liu", "Xin Zhou", "Hao Su", "Liming Shang", "Yizhen Gong", "Xiaowei Song", "Tao Peng", "Xinping Ye"], "journal": "Oncology reports", "year": 2019, "abstract": "The aim of the present study was to investigate the diagnostic and prognostic value of Wingless‑type MMTV integration site (WNT) gene family expression in patients with hepatitis B virus (HBV)‑related hepatocellular carcinoma (HCC). The clinical data of the patients and gene expression levels were downloaded from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. Receiver operating characteristic curve analysis was used to investigate the diagnostic value of WNT genes. Cox proportional hazard regression analysis and Kaplan‑Meier survival analysis were performed to evaluate the association of WNT gene expression level with overall survival (OS) and recurrence‑free survival (RFS). A nomogram was constructed for the prediction of prognosis. Hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated. Diagnostic receiver operating characteristic curve analysis suggested that WNT2 had a high diagnostic value, with an area under the curve (AUC) of >0.800 (P<0.0001, AUC=0.810, 95% CI: 0.767‑0.852). Survival analysis indicated that the expression level of WNT1 was significantly associated with OS and RFS (adjusted P=0.033, adjusted HR=0.607, 95% CI: 0.384‑0.960; and adjusted P=0.007, adjusted HR=0.592, 95% CI: 0.404‑0.868, respectively). In the TCGA validation cohort, we also observed that WNT2 was significantly differentially expressed between HCC tissues and adjacent non‑tumor tissues, and WNT1 was associated with both the OS and RFS of HCC. Therefore, through the GSE14520 HBV‑related HCC cohort we concluded that WNT2 may serve as a diagnostic biomarker and WNT1 may serve as a prognostic biomarker. These results may also be extended to TCGA HCC verification cohort.", "claims": [{"id": "claim-method", "statement": "Cox proportional hazard regression analysis and Kaplan-Meier survival analysis with log-rank tests were used to evaluate the association between WNT family gene expression, OS and recurrence-free survival (RFS).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31322232", "locator": "sections[7] \"Survival analysis\" paragraph 2", "quote": "Cox proportional hazard regression analysis and Kaplan-Meier survival analysis with log-rank tests were used to evaluate the association between WNT family gene expression, OS and recurrence-free survival (RFS)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31322232", "locator": "sections[24] \"Availability of data and materials\" paragraph 2", "quote": "The datasets analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31322232"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE14520"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE14520"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:31322232", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3892/or.2019.7224", "sha256": 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