Lyn regulates epithelial-mesenchymal transition in CS-exposed model through Smad2/3 signaling.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31477108", "kind": "science-record", "revision": 8, "content_hash": "sha256:47546a0fd7a6f3ae27b09f63fde967d387d343dd46dd70a8a73b089026f89516", "created_at": "2026-11-01T15:40:49Z", "updated_at": "2026-11-01T22:44:10Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Lyn regulates epithelial-mesenchymal transition in CS-exposed model through Smad2/3 signaling.", "doi": "10.1186/s12931-019-1166-z", "pmid": "31477108", "authors": ["Xiaobo Liang", "Xiang He", "Yin Li", "Junyi Wang", "Dehong Wu", "Xiefang Yuan", "Xiaoyun Wang", "Guoping Li"], "journal": "Respiratory research", "year": 2019, "abstract": "BACKGROUND: Chronic obstructive pulmonary disease (COPD) is characterized by airflow limitation that is progressive and not fully reversible. Cigarette smoking is one of the most commonly and important risk factors for COPD, which contributes to airway remodeling, the outstanding pathological changes in COPD. One potential mechanism which might be important for airway remodeling is the process called epithelial-mesenchymal transition (EMT). However, the underlying molecular mechanisms of EMT in CS-induced COPD are still poorly understood.\nMETHODS: Two Gene Expression Omnibus (GEO) datasets (GSE108134 and GSE5058) were combined to identify the key genes involved in COPD. Then, single-gene analysis of Lyn was performed. Lyn expression was confirmed in patients with COPD. 16HBE cells were treated with cigarette smoking extracts (CSE). Wild type (WT) C57BL/6 J mice and Lyn+/+ transgenic mice were exposed to CSE to establish CS-exposed model. Pathological changes were observed by hematoxylin-eosin staining. The expression levels of EMT markers were examined by using western blot and immunofluorescence. The expression and phosphorylation levels of Lyn and Smad2/3 were detected as well.\nRESULTS: The gain of mesenchymal markers vimentin and α-SMA with a concomitant loss of E-cadherin was observed in both in vivo and in vitro studies. Meanwhile, cigarette smoking extracts (CSE) induced EMT in 16HBE cells in a time- and dose- dependent manner. Furthermore, by analyzing GEO datasets and using molecular methods, we explored a kinase, Lyn, its expression correlated with the expression of E-cadherin, vimentin and α-SMA in CS-exposed model. Moreover, we found that EMT induced by CSE was regulated by activated Lyn through phosphorylation of Smad2/3.\nCONCLUSIONS: In summary, we found that Lyn regulates epithelial-mesenchymal transition in CS-exposed model through Smad2/3 signaling. As a kinase, Lyn is \"druggable\", and might provide a therapeutic opportunity for targeting EMT. Therefore, our research might provide a new method to treat COPD by targeting Lyn kinase specifically.", "claims": [{"id": "claim-method", "statement": "Multiple group comparisons were analyzed using a one-way ANOVA.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31477108", "locator": "sections[11] \"Statistical analysis\" paragraph 2", "quote": "Multiple group comparisons were analyzed using a one-way ANOVA."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31477108", "locator": "sections[25] \"notes\" paragraph 1", "quote": "The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31477108"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE108134"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE108134"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:31477108", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1186/s12931-019-1166-z", "sha256": 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Meanwhile, cigarette s", "d. (Chongqing, China). Lyn+/+ TG mice and WT mice were maintained in a specific pathogen-free facil"], "order_basis": "paper_text_order:946", "id": "assay:1"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["levels of EMT markers were examined by using western blot and immunofluorescence. The expression and p", "described [22]. Genotyping was confirmed by western blotting. Wild type (WT) C57BL/6 J mice at 8 to 10 we", "P = 0.393). To validate the microarray data, western blot was applied. It also showed that the protein"], "order_basis": "paper_text_order:1128", "id": "assay:2"}, {"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["kers were examined by using western blot and immunofluorescence. The expression and phosphorylation levels o"], "order_basis": "paper_text_order:1145", "id": "assay:3"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Wang [22]. Cell culture, viral infection and transfection Human Lyn cDNA was amplified and cloned into", "he vector expressing Lyn was constructed and transfected into human embryo kidney cells 293 T cells t", "ing 10% fetal bovine serum. 16HBE cells were transfected with recombinant lentivirus containing Lyn o", "anufacturer’s instructions. 16HBE cells were transfected with lentivirus at a multiplicity of infecti"], "order_basis": "paper_text_order:2843", "id": "assay:4"}, {"role": "data_generation", "definition_id": "microarray", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["n of E-cadherin (P = 0.393). To validate the microarray data, western blot was applied. It also show"], "order_basis": "paper_text_order:5556", "id": "assay:5"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Multiple group comparisons were analyzed using a one-way ANOVA."], "order_basis": "consumes_earlier_output", "id": "assay:6"}]}