Comparative study of excretory-secretory proteins released by Schistosoma mansoni-resistant, susceptible and naïve Biomphalaria glabrata.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31521183", "kind": "science-record", "revision": 8, "content_hash": "sha256:2a74cdebbe2655b84391e587db2138e1ecf0427542f1ddd7280a9dc637579edf", "created_at": "2026-11-01T15:40:49Z", "updated_at": "2026-11-01T22:44:11Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Comparative study of excretory-secretory proteins released by Schistosoma mansoni-resistant, susceptible and naïve Biomphalaria glabrata.", "doi": "10.1186/s13071-019-3708-0", "pmid": "31521183", "authors": ["Conor E Fogarty", "Min Zhao", "Donald P McManus", "Mary G Duke", "Scott F Cummins", "Tianfang Wang"], "journal": "Parasites & vectors", "year": 2019, "abstract": "BACKGROUND: Schistosomiasis is a harmful neglected tropical disease caused by infection with Schistosoma spp., such as Schistosoma mansoni. Schistosoma must transition within a molluscan host to survive. Chemical analyses of schistosome-molluscan interactions indicate that host identification involves chemosensation, including naïve host preference. Proteomic technique advances enable sophisticated comparative analyses between infected and naïve snail host proteins. This study aimed to compare resistant, susceptible and naïve Biomphalaria glabrata snail-conditioned water (SCW) to identify potential attractants and deterrents.\nMETHODS: Behavioural bioassays were performed on S. mansoni miracidia to compare the effects of susceptible, F1 resistant and naïve B. glabrata SCW. The F1 resistant and susceptible B. glabrata SCW excretory-secretory proteins (ESPs) were fractionated using SDS-PAGE, identified with LC-MS/MS and compared to naïve snail ESPs. Protein-protein interaction (PPI) analyses based on published studies (including experiments, co-expression, text-mining and gene fusion) identified S. mansoni and B. glabrata protein interaction. Data are available via ProteomeXchange with identifier PXD015129.\nRESULTS: A total of 291, 410 and 597 ESPs were detected in the susceptible, F1 resistant and naïve SCW, respectively. Less overlap in ESPs was identified between susceptible and naïve snails than F1 resistant and naïve snails. F1 resistant B. glabrata ESPs were predominately associated with anti-pathogen activity and detoxification, such as leukocyte elastase and peroxiredoxin. Susceptible B. glabrata several proteins correlated with immunity and anti-inflammation, such as glutathione S-transferase and zinc metalloproteinase, and S. mansoni sporocyst presence. PPI analyses found that uncharacterised S. mansoni protein Smp_142140.1 potentially interacts with numerous B. glabrata proteins.\nCONCLUSIONS: This study identified ESPs released by F1 resistant, susceptible and naïve B. glabrata to explain S. mansoni miracidia interplay. Susceptible B. glabrata ESPs shed light on potential S. mansoni miracidia deterrents. Further targeted research on specific ESPs identified in this study could help inhibit B. glabrata and S. mansoni interactions and stop human schistosomiasis.", "claims": [{"id": "claim-method", "statement": "A two-way ANOVA test was used to calculate P-values and evaluate the significance of behavioural modifications in response to pH-neutral water, susceptible and F1 resistant SCW.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31521183", "locator": "sections[5] \"Schistosoma mansoni miracidia isolation and behavioural bioassay\" paragraph 4", "quote": "A two-way ANOVA test was used to calculate P-values and evaluate the significance of behavioural modifications in response to pH-neutral water, susceptible and F1 resistant SCW."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [41] partner repository with the dataset identifier PXD015129. 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Other datasets analysed in this study are included in this published article and its additional files."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31521183"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": [], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": [], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:obtain_source_data blocked on data:accession_absent"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:31521183", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1186/s13071-019-3708-0", "sha256": "sha256:413b5a54eb9818bf16493e6ffb965da556076cef83ee36ea2845fe6ea88257c7", "bytes": 74161, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1186/s13071-019-3708-0", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC6744689", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "asset:table:pmid:31521183:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:86287af3937d2519686dfd365e057dfe90b71964966bb83d1e89f3725c28a838", "bytes": 1444, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[14]/table-wrap[1]#Tab1", "version": null, "note": "High confidence non-redundant ESPs ([− 10lgP] > 30) with putative anti-pathogen function identified in resistant B. glabrata SCW. Details of coverage, peptide match number and BLAST confidence (e-value) are shown", "structure": {"pmcid": "PMC6744689", "section_index": 14, "section_title": "Biomphalaria glabrata ESP annotation and gene ontology analysis", "row_count": 12, "column_count": 8}, "table": {"label": "Table 1", "caption": "High confidence non-redundant ESPs ([− 10lgP] > 30) with putative anti-pathogen function identified in resistant B. glabrata SCW. Details of coverage, peptide match number and BLAST confidence (e-value) are shown", "rows": [["Description", "− 10lgP", "Coverage (%)a", "No. of peptides", "No. uniqueb", "Avg. mass", "BLASTp e-value", "Accession No."], ["Leukocyte elastase inhibitor-like", "73.93", "15", "2", "1", "15593", "1.01E−98", "BGLB007529-PB"], ["Haemoglobin type 1", "69.56", "12", "4", "1", "20380", "4.65E−130", "BGLB026333-PA"], ["Superoxide dismutase [Cu-Zn]", "59.07", "14", "2", "2", "16447", "4.39E−104", "BGLB000035-PA"], ["Adenosine deaminase CECR1", "58.36", "8", "2", "2", "25076", "3.34E−159", "BGLB033953-PA"], ["Glutathione peroxidase-like", "50.23", "12", "2", "2", "22859", "1.85E−151", "BGLB008980-PC"], ["Heat-shock 70 kDa protein cognate 4", "45.56", "5", "4", "4", "71177", "0", "BGLB007783-PB"], ["Enolase-phosphatase E1", "42.13", "13", "2", "2", "19313", "4.05E−119", "BGLB029757-PA"], ["Peroxiredoxin 1", "39.55", "4", "1", "1", "27567", "0", "BGLB000120-PA"], ["Rho GDP-dissociation inhibitor 1", "32.87", "4", "1", "1", "23176", "2.75E−143", "BGLB011409-PB"], ["Carboxypeptidase B", "32.85", "12", "1", "1", "12298", "2.51E−73", "BGLB037596-PA"], ["Putative tyrosinase-like protein tyr-1", "31.61", "2", "1", "1", "67700", "0", "BGLB030046-PA"]], "row_count": 12, "column_count": 8, "numeric_cells": 83, "header_rows": 1, "locator": "section[14]/table-wrap[1]#Tab1"}}, {"id": "asset:table:pmid:31521183:2", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:ade14208a1415dc843274206ff4b2cb0fc0643e0eeb91bc4741c0e321e8206c3", "bytes": 1902, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[14]/table-wrap[2]#Tab2", "version": null, "note": "High confidence non-redundant ESPs (-10lgP > 30) identified in the susceptible B. glabrata SCW. 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Details of sequence coverage, average mass, number of peptides and unique peptides and accession names are shown", "structure": {"pmcid": "PMC6744689", "section_index": 15, "section_title": "Schistosoma mansoni proteins identified from susceptible SCW of B. glabrata", "row_count": 14, "column_count": 8}, "table": {"label": "Table 3", "caption": "A list of non-redundant S. mansoni ESPs identified in susceptible SCW. 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