Liver proteome dataset of Sparus aurata exposed to low temperatures.

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Gilthead sea bream juveniles were reared in Recirculating Aquaculture Systems (RAS) and exposed to a temperature ramp made of two phases of four weeks each: a Cooling phase from 18 °C (t0) to 11 °C (t1) and a Cold Maintenance phase at 11 °C (t1-t2) in a 8 week feeding trial. At the end of the experiment, sea bream livers were collected and analyzed with a shotgun proteomics approach based on filter-aided sample preparation followed by tandem mass spectrometry, peptide identification carried out using Sequest-HT as search engine within the Proteome Discoverer informatic platform, and label-free differential analysis. The mass spectrometry data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD011059 (Vizcaíno et al., 2016; Deutsch et al., 2017; Perez-Riverol et al., 2016). The dataset described here is also related to the research article entitled \"Liver proteomics of gilthead sea bream (Sparus aurata) exposed to cold stress\" (Ghisaura et al., 2019).", "claims": [{"id": "claim-method", "statement": "To evaluate the statistical significance of differential protein abundance between logarithmized (normally distributed) NSAF values, a student's t-test (two-sample comparison, p < 0.05) was applied.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31528672", "locator": "sections[8] \"Data analysis\" paragraph 3", "quote": "To evaluate the statistical significance of differential protein abundance between logarithmized (normally distributed) NSAF values, a student's t-test (two-sample comparison, p < 0.05) was applied."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data is within this article and available via the ProteomeXchange Consortium, dataset identifier PXD011059", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31528672", "locator": "sections[1] \"table-wrap\" paragraph 1", "quote": "Data is within this article and available via the ProteomeXchange Consortium, dataset identifier PXD011059"}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31528672"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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