Weighted gene coexpression analysis indicates that PLAGL2 and POFUT1 are related to the differential features of proximal and distal colorectal cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31638246", "kind": "science-record", "revision": 7, "content_hash": "sha256:0dcae8ba7b00060eed13059d914876b7f96f72f6eeb86538027ac9b3921c0112", "created_at": "2026-11-01T15:40:50Z", "updated_at": "2026-11-01T22:44:16Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Weighted gene coexpression analysis indicates that PLAGL2 and POFUT1 are related to the differential features of proximal and distal colorectal cancer.", "doi": "10.3892/or.2019.7368", "pmid": "31638246", "authors": ["Yiming Lv", "Binbin Xie", "Bingjun Bai", "Lina Shan", "Wenqian Zheng", "Xuefeng Huang", "Hongbo Zhu"], "journal": "Oncology reports", "year": 2019, "abstract": "In the current era of precision medicine, there is a general consensus that the anatomical site is an important factor in the management of colorectal cancer (CRC). To investigate the underlying molecular mechanisms between proximal and distal CRC and to identify the responsible genes, we analyzed the gene expression patterns of colorectal tumors from two microarray datasets, GSE39582 and GSE14333, on the NCBI Gene Expression Omnibus and the RNA‑seq data from TCGA. Weighted coexpression network analysis (WGCNA) was applied to construct a gene coexpression network. The red module in GSE39582 and the dark‑gray module from the TCGA dataset were found to be highly correlated with the anatomical site of CRC. A total of 12 hub genes were found in two datasets, 2 of which PLAG1 like zinc finger 2 (PLAGL2) and protein O‑fucosyltransferase 1 (POFUT1) were common and upregulated in tumor samples in CRC. The module with the highest correlation provided references that will help to characterize the difference between left‑sided and right‑sided CRC. The survival analysis of PLAGL2 and POFUT1 expression revealed differences between proximal and distal CRC. Gene set enrichment analysis based on those two genes provided similar results: GPI anchor biosynthesis and peroxisome and selenoamino acid metabolism. PLAGL2 and POFUT1, which have the highest correlation with tumor location, may serve as biomarkers and therapeutic targets for the precise diagnosis and treatment of CRC in the future.", "claims": [{"id": "claim-method", "statement": "Kaplan-Meier analysis and log-rank test were performed to evaluate the association between hub gene expression and patient survival in left- and right-sided CRC, respectively.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31638246", "locator": "sections[10] \"Survival analysis\" paragraph 2", "quote": "Kaplan-Meier analysis and log-rank test were performed to evaluate the association between hub gene expression and patient survival in left- and right-sided CRC, respectively."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31638246", "locator": "sections[24] \"Availability of data and materials\" paragraph 2", "quote": "The datasets analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31638246"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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