CRYAB promotes osteogenic differentiation of human bone marrow stem cells via stabilizing β-catenin and promoting the Wnt signalling.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31638302", "kind": "science-record", "revision": 8, "content_hash": "sha256:9cb320a78c100a329057fb5855ed5c121696d19dea0a9b547c70c15ca7764370", "created_at": "2026-11-01T15:40:50Z", "updated_at": "2026-11-01T22:44:16Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "CRYAB promotes osteogenic differentiation of human bone marrow stem cells via stabilizing β-catenin and promoting the Wnt signalling.", "doi": "10.1111/cpr.12709", "pmid": "31638302", "authors": ["Bin Zhu", "Feng Xue", "Guangyi Li", "Changqing Zhang"], "journal": "Cell proliferation", "year": 2020, "abstract": "OBJECTIVES: The osteogenesis differentiation of human bone marrow stem cells (BMSCs) is essential for bone formation and bone homeostasis. In this study, we aim to elucidate novel molecular targets for bone metabolism diseases.\nMATERIALS AND METHODS: The dataset GSE80614 which includes mRNA expression profile during BMSCs osteogenic differentiation was obtained from the GEO database (https://www.ncbi.nlm.nih.gov/geo/). The osteogenic differentiation of BMSCs was measured by ALP staining, AR staining and expression of osteogenic markers in vitro. For in vivo assay, we seeded BMSCs onto beta-tricalcium phosphate (β-TCP) and transplanted them into muscle pockets of nude mice. Luciferase assay, co-immunoprecipitation assay and in vitro ubiquitination assay were carried out to investigate the molecular mechanism.\nRESULTS: We found that α-B-crystallin (CRYAB) expression was elevated during the process of BMSCs osteogenic differentiation. Further studies showed that upregulation of CRYAB significantly enhanced the osteogenic differentiation, while downregulation of CRYAB suppressed it. CRYAB regulated BMSCs osteogenic differentiation mainly through the canonical Wnt/β-catenin signalling. In addition, we found that CRYAB could physically interact with β-catenin and protect it from ubiquitination and degradation, which stabilized β-catenin and promoted the Wnt signalling.\nCONCLUSIONS: The present study provides evidences that CRYAB is an important regulator of BMSCs osteogenic differentiation by protecting β-catenin from ubiquitination and degradation and promoting the Wnt signalling. It may serve as a potential therapeutic target for diseases related to bone metabolism.", "claims": [{"id": "claim-method", "statement": "Two‐tailed Student's t test was used to evaluate the statistical differences between two groups, and one‐way analysis of variance (ANOVA) was used to evaluate the statistical differences among more than two groups. The data that support the findings of this study are openly available in GEO database at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE80614.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31638302", "locator": "sections[11] \"Statistical analysis\" paragraph 2", "quote": "Two‐tailed Student's t test was used to evaluate the statistical differences between two groups, and one‐way analysis of variance (ANOVA) was used to evaluate the statistical differences among more than two groups."}, {"asset": "source:pmid:31638302", "locator": "sections[25] \"DATA AVAILABILITY STATEMENT\" paragraph 2", "quote": "The data that support the findings of this study are openly available in GEO database at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE80614."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data that support the findings of this study are openly available in GEO database at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE80614.66", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31638302", "locator": "sections[25] \"DATA AVAILABILITY STATEMENT\" paragraph 2", "quote": "The data that support the findings of this study are openly available in GEO database at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE80614.66"}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31638302"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE80614"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "blocked", "blocked_on": "method:student_t", "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE80614"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:run_analysis blocked on method:student_t"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:31638302", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1111/cpr.12709", "sha256": "sha256:fbf437fcaf9313d25779efa864f78ded53f8a443787f10f7ccd997cb1ac3371a", "bytes": 43922, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1111/cpr.12709", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC6985673", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE80614", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE80614", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE80614", "version": null, "note": "The data that support the findings of this study are openly available in GEO database at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE80614.66", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The data that support the findings of this study are openly available in GEO database at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE80614.66", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM2131663", "GSM2131664", "GSM2131665", "GSM2131666", "GSM2131667", "GSM2131668", "GSM2131669", "GSM2131670", "GSM2131671", "GSM2131672", "GSM2131673", "GSM2131674", "GSM2131675", "GSM2131676", "GSM2131677", "GSM2131678", "GSM2131679", "GSM2131680", "GSM2131681", "GSM2131682", "GSM2131683", "GSM2131684", "GSM2131685", "GSM2131686", "GSM2131687", "GSM2131688", "GSM2131689", "GSM2131690", "GSM2131691", "GSM2131692", "GSM2131693", "GSM2131694", "GSM2131695", "GSM2131696", "GSM2131697", "GSM2131698", "GSM2131699", "GSM2131700", "GSM2131701", "GSM2131702", "GSM2131703", "GSM2131704", "GSM2131705", "GSM2131706", "GSM2131707", "GSM2131708", "GSM2131709", "GSM2131710", "GSM2131711", "GSM2131712", "GSM2131713", "GSM2131714", "GSM2131715", "GSM2131716", "GSM2131717", "GSM2131718", "GSM2131719", "GSM2131720", "GSM2131721", "GSM2131722", "GSM2131723", "GSM2131724", "GSM2131725", "GSM2131726", "GSM2131727", "GSM2131728", "GSM6758338", "GSM6758339", "GSM6758340"], "sample_count": 69, "group_sizes": null, "group_sizes_basis": null, "platform": "6947", "taxon": "Homo sapiens", "title": "Gene expression analyses of the differentiating hMSC into Osteoblasts and Adipocytes.", "resolution_checked_at": "2026-11-01T15:39:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE80614 not downloaded", "asset:data:geo:GSE80614 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": "group_comparison", "specific": "student_t_test", "label": "Group comparison", "dsl_operations": [], "missing_operations": ["student_t"], "expressible": false, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 1.0, "band": "reordered", "quote": "Two‐tailed Student's t test was used to evaluate the statistical differences between two groups, and one‐way analysis of variance (ANOVA) was used to evaluate the statistical differences among more than two groups. The data that support the findings of this study are openly available in GEO database at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE80614."}}, {"id": "method-2", "family": "group_comparison", "specific": "anova", "label": "Group comparison", "dsl_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 0.8, "band": "high", "quote": "analysis of variance"}}], "targets": [], "gaps": ["method.student_t"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": false, "required_operations": [], "missing_operations": ["student_t"], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: student_t_test."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE80614"], "resolved_accessions": ["GSE80614"], "statements": ["The data that support the findings of this study are openly available in GEO database at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE80614.66"], "basis": "The paper names GSE80614 in GEO database and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": [], "assets": ["source:pmid:31638302", "data:geo:GSE80614"], "steps": []}, "must_build": {"dsl_operations": [{"operation": "student_t", "unlocks_papers": null, "priority": null}], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE80614"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-10-31T18:16:32Z", "independence": "single_reader"}, "evidence_check": {"method": "reordered", "availability": "verbatim"}, "calls": 3, "usage": {"prompt_tokens": 35365, "completion_tokens": 1215, "cached_tokens": 776}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:31638302", "type": "paper", "label": "pmid:31638302"}, {"id": "source:pmid:31638302", "type": "manuscript", "label": "PMID 31638302 full text"}, {"id": "data:geo:GSE80614", "type": "dataset", "label": "GEO GSE80614"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:31638302", "type": "paper", "label": "pmid:31638302"}, {"id": "source:pmid:31638302", "type": "manuscript", "label": "PMID 31638302 full text"}, {"id": "data:geo:GSE80614", "type": "dataset", "label": "GEO GSE80614"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-31638302", "type": "extraction", "used": ["source:pmid:31638302"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE80614", "type": "accession_resolution", "used": ["data:geo:GSE80614"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:31638302", "parent_hash": "sha256:437eca57993676fdfc9cc3b7785bbe571fbeda706e8a36db314df003e6129f02", "fork_of": null, "generation": 7}, "gaps": ["asset:data:geo:GSE80614 licence not read", "asset:data:geo:GSE80614 not downloaded", "execution.not_run", "method.student_t", "review.human_critique", "review.independent_reader", "review.machine_critique", "step:run_analysis blocked on method:student_t"], "assays": [{"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["pression of osteogenic markers in vitro. For in vivo assay, we seeded BMSCs onto beta-tricalcium", "tor. Animal studies Eight‐week‐old male nude mice were employed for animal studies in this experime", "studies in this experiment, and all of these mice were kept in a specific‐pathogen‐free condition.", "on the posterior limb of the mice. Forty‐two mice were divided into seven groups, namely sh‐Control"], "order_basis": "paper_text_order:645", "id": "assay:1"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Two‐tailed Student's t test was used to evaluate the statistical differences between two groups, and one‐way analysis of variance (ANOVA) was used to evaluate the statistical differences among more than two groups. The data that support the findings of this study are openly available in GEO database at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE80614."], "order_basis": "consumes_earlier_output", "id": "assay:2"}]}